Other Applications

• Dispensing Cells and Beads
• Maintaining Sample Integrity
• Elimination of Serial Dilutions
• General Acoustic Droplet Ejection

Dispensing Cells and Beads

• Automated Colony Spotting [applications note]
• A-180 Automated Dispensation of Yttrium Oxide SPA Imaging Beads [applications note]
• Acoustic Droplet Ejection and Laser Scanning Platforms Enable High-Throughput Cell Dispensing and Quantitation S. Miller, J. Shieh, S. Pickett, Labcyte and Blueshift Technologies [poster]
• Automated Dispensation of Yttrium Oxide SPA Imaging Beads, into 1536-Well Plates using Deerac Fluidics' Equator HTS Pipetting System [poster]
• High Content Screening with Sub-Wavelength Optical Imaging of Cells Transferred Acoustically into Sub-Microliter Wells, A. Cabasug, M. Mutz, R. Stearns [poster]

Maintaining Sample Integrity

• Applying the Labcyte MicroClime® Lid in Biological Assays to Improve Data Quality and Quantity, Adrian Freeman, M. Fagura, G. Bellfield & J. Unitt, AstraZeneca R&D Charnwood, UK, [poster]
• Compound Preservation in Closed Loop Screening [poster]
• Hydration Free, Automation-Friendly Alternative for Compound Library Storage [poster]
• Optimum Conditions for Thermal Sealed Plates with Acoustic Liquid Transfers J. Shieh, M. Gomez, J. Barco, I. Yates. [poster]
• Putting a Lid on Edge Effects: Protecting Compound Libraries with Lid-Based Environmental Control [poster]
• DMSO Compound Library Volume Measurement and Hydration Monitoring in Storage Tubes and Microplates, presentation, LabFusion 2004 [Presentation]

Elimination of Serial Dilutions

• Elimination of Serial Dilution [poster]
• Elimination of Serial Dilutions -Improving Throughput in High-Density Format Microplates While Eliminating Cost and Improving Results [poster]
• New Tool for Automating Serial Dilutions for Activity ConfirmationExperiments, J. Cesarek and D. Nie, AstraZeneca [poster]

General acoustic droplet ejection (ADE)

• Acoustic Droplet Ejection "Touchless" Transfer This note describes acoustic droplet ejection (ADE) and it's background [applications note]
• How Long Will A Survey Last When Performing Acoustic Droplet Ejections? [applications note]
• A Sound Idea by Laura Cutland, San Jose Business Journal, April 22, 2005 [Article]
• Acoustic droplet ejection in drug discovery, E Heron, R Ellson, J Olechno, Drug Plus International, April/May 2006, pp 22-25 [Article]
• Acoustic Droplet Ejection Technology, J Olechno, R Stearns, R Ellson, E Heron, Innovations in Pharmaceutical Technology, April, 2006, pp 40-43 [Article]
• Automating Compound Management Systems, Catherine Shaffer, Drug Discovery & Development, August 2005, pp 36-41 [Article]
• Continued Miniaturisation of Assay Technologies Drives Market for Nanolitre Dispensing, Drug Discovery World, Summer 2004, pages = 43-54
• Transfer of Low Nanoliter Volumes between Microwell Plates Using Focused Acoustics-Automation Considerations, Journal of the Association of Laboratory Automation, 8 (5) 2003 pp34-39 [Article]
• Debunking the Myth: Validation of Fluorescein for Testing the Precision of Nanoliter Dispensing, D L Harris and M Mutz, JALA, 11(4), 233-239, 2006 [Article]
• Improving IC50 Results with Acoustic Droplet Ejection, J Olechno, J Shieh, R Ellson, JALA, 11(4), 240-246, 2006
• In situ DMSO Hydration Measurements of HTS Compound Libraries, Ellson et al, Combinatorial Chemistry & High Throughput Screening, 8(6), September 2005, pp 489-498 Click here to read the abstract of the paper [Article]
• Labcyte Inc: Company Interview, The Wall Street Transcript, November 2003 [Article]
• Leaps in Dispensing Change Liquid Handling Landscape by Sean Keating, Managing Editor, Drug Discovery & Development, January, 2005, 46-48 [Article]
• Marketing in Science and the Science of Marketing: Hugh Kennedy interviews Elaine Heron, CEO, Labcyte, Hugh Kennedy, September 2007 [Article]
• Miniaturization and Low Volume Liquid Handling Take the Lead in Lab Automation, B Tulsi, Bioscience Technology [Article]
• Nano to Next Gen: Automation Gets Personal, J Perkel, Science, Vol 319, Issue 5861, 345-347, January 18, 2008
• Picoliter: Company Profile, American Laboratory, August 2003 [Article]
• Picoliter: enabling precise transfer of nanoliter and picoliter volumes, Drug Discovery Today 7 (5) (2002) pp S32-S34 [Article]
• Precision Nanoliter Aqueous Transfer, J Shieh, S Pickett, J Olechno, GEN News, Vol 27 No 9, May 1 2007 [Article]
• Serial vs Direct Dilution Time to apply new thinking to IC50 determination and dose response analysis? J Comley (HTStec Inc), Drug Discovery World, Spring 2007, 36-50 [Article]
• Sound Advice: Acoustic technologies facilitate ultra-low volume liquid handling, Today's Chemist at Work, October 2003 [Article]
• Sound Effects American Laboratory, December 2004, pp 26-30 [Article]
• The Effects of Small Molecule and Protein Solutes on Acoustic Drop Ejection, J Shieh, R N Ellson and J Olechno, JALA, 11(4), 227-232, 2006 [Article]
• Touchless Liquid Transfer for the Life Sciences Genetic Engineering News, November 15, 2004, 60-61 Rich Ellson interview by Nina Bell of Lab-on-a-chipcom, August 2004 [Article]
• Echo 550 (Click Here to Open) [Movie]
• Movie of acoustic droplet ejection into a well filled with aqueous buffer. This movie shows that when the dense solution of DMSO is ejected into an inverted solution of buffer, the DMSO stays at the meniscus until the plate is returned to the normal position. The DMSO solution then diffuses gradually rather than as a bolus. [Movie]
• Movie of pin tools adding DMSO to PBS solutions courtesy of Dr. Joerg Dreessen of Novartis. This movie shows the addition of DMSO (containing a dye for easy visualization) into a buffer using pin tools. The dense DMSO drops as an undiluted bolus to the bottom of the well. Cells adherent to the bottom of the cell are exposed to injuriously high levels of DMSO until the solution completely disperses. [Movie]
• Stroboscopic movie of 2.5 nL droplets being ejected from a single well. This movie captures many individual droplets into one, easy to understand sequence. [Movie]
• Best Practices for Assessing Nanoliter Transfer Performance for Labcyte Echo Liquid Handlers Using the Artel MVS [poster]
• Debunking the Myth - Using Fluorescein in Analytical Measurements of Fluid Transfers [poster]
• Impact of Compound Concentration in Solutions on Acoustic Detection and Transfers [poster]
• Integrating Acoustic Droplet Ejection into the Laboratory Environment [poster]
• Keeping DMSO Concentration Below 0.5% to Minimize its Effect in HTS Assays [poster]
• [POSTER] Low Nanoliter Acoustic Transfer of Aqueous Fluids with High Precision and Accuracy
• Nanoliter Transfers in 1536-Well Format Using Acoustic Droplet Ejection [poster]
• Pharmacological Evaluation of Different Compound Dilution and Transfer Paradigms on an Enzyme Assay in Low Volume 384-well Format, T. Spicer, Y. Fitzgerald, N. Burford, S. Matson, M. Chatterjee, M. Gilchrist, J. Myslik and J. O'Connell, Bristol-Myers Squibb [poster]
• Testing the Precision of Acoustic Droplet Ejection - Measuring CVs for 5 Nanoliter Transfers [poster]
• The Microliter Assay Limbo [poster]
• Low and Sub Nanoliter Dispensing of Volatile Reagents for Sample Preparation and Crystallization, presentation, SmallTalk 2003 [Presentation]
• Managing Compound Library Health Through Solvent Restoration, (7MB file) 8th International MipTec Conference, May 2005 [Presentation]
• Nanoliter and Picoliter Liquid Handling for Life Science Applications, paper, Nanotech/Biotech Convergence 2003 [Presentation]
• Non-Invasive Liquid and Live Cell Transfers for HTS, presentation, Lab Automation, 2003 [Presentation]
• High Density IC50 Analyses Using the Echo 550 Liquid Handler [Webinar]