Cost-effective, high-throughput RT-qPCR
Eliminate costly and laborious preparation steps to enable high throughput RT-qPCR analysis of endogenous gene expression. The Echo Liquid Handler combined with one-step reagents, improved detection systems and automated plate handling makes cost-effective, high-throughput RT-qPCR a reality.
RT-qPCR is arguably the most sensitive technology available for measuring endogenous gene expression. However, despite having clear advantages over reporter-based methods, rigorous sample preparation steps and reagent costs have prohibited the use of RT-qPCR when high-throughput analysis is required. Overcoming this, use of the Echo Liquid Handler to transfer cell lysates and novel one-step qPCR reagents has demonstrated a tremendous reduction in reaction volumes while improving data quality. With Echo Liquid Handlers reaction volumes can be reduced to 250 nL or less in 384-well or 1536-well PCR plates. To expand these benefits even further, the Access Laboratory Workstation incorporates the Echo Liquid Handler into a turnkey, automated solution for high throughput gene expression—cell lysates direct to PCR in a single step.