Home

Applications

APPLICATIONS OVERVIEW
DRUG DISCOVERY
ADME-Tox
Biochemical Assays
Cell-based Assays
HTS and Secondary Screening
Microsampling
Protein Crystallography
RNAi
Sample Management
CANCER RESEARCH
Personalized Medicine
Functional Screening / DSRT
RNAi
Sequencing
Single-cell Genomics
GENOMIC RESEARCH
Synthetic Biology
Gene Expression
Genotyping
qPCR
Sequencing
Single-cell Genomics

Products

LIQUID HANDLING
LIQUID HANDLERS OVERVIEW
Echo® 525 Liquid Handler
Echo® 555 Liquid Handler
Echo® 550 Liquid Handler
Echo® 520 Liquid Handler
AUTOMATION
AUTOMATION OVERVIEW
Access™ Laboratory Workstation
POD™ Automation Platform
Tempo™ Automation Control Software
SOFTWARE APPLICATIONS
SOFTWARE OVERVIEW
Echo® Array Maker
Echo® Cherry Pick
Echo® Combination Screen
Echo® Dose-Response
Echo® Plate Audit
Echo® Plate Reformat
CONSUMABLES
CONSUMABLES OVERVIEW
Echo® Qualified Microplates
Labcyte® Assay Microplates
MicroClime® Environmental Lid
Echo® Qualified Reservoir

Technology

Echo® Acoustic Liquid Handling
Dynamic Fluid Analysis™
Direct Dilution

News / Events

Labcyte – San Diego 2017
Labcyte BLOG
Upcoming Events
Press Releases
Labcyte in the News
Labcyte Community

Resources

JALA Special Issue
Publications
Application Notes
Posters
Webinars
Videos
Customer Profiles
Core Labs

Support

Brochures
User Guides*
Quick Start Guides*
Specification Sheets
Site Prep Guides
Integration Models
Service and Maintenance
Request Information

Company

About Us
Echo® Acoustic Technology
Leadership Team
Careers
Contact and Location
Privacy Policy

Microsampling

for pharmacokinetic (PK) and pharmacodynamic (PD) studies

HOME | APPLICATIONS | Microsampling

Reduce Costs and Use Fewer Animals in PK and PD Studies with Echo® Liquid Handlers and Serial Microsampling

High precision, low volume fluid transfers by the Echo Liquid Handler make it possible for researchers to perform serial sampling for pharmacokinetic (PK) and pharmacodynamic (PD) studies — dramatically reducing study costs and animal use while increasing data quality.

Benefits of Microsampling on the Echo Liquid Handler


  • Microsampling better addresses the 3 R’s of animal welfare: Replace, Refine, Reduce
  • Use as little at 1 µL of plasma from mice vs 50 µL with no loss of data quality
  • Less stress on test animals
  • Enables alternative blood collection that does not require surgery
  • Less animal mortality
  • Decrease study costs
  • Save up to 80% of study costs, 89% of animal costs, and 75% of test compound used*
  • Use of fewer animals in study
  • Increased data quality
  • Removes the animal to animal variability seen in composite collection studies by decreasing the number of subjects used within a study
  • Less disturbance to critical physiological parameters such as heart and respiratory rate
  • Utilize the same animals for PK and PD studies for better correlation between drug level and drug action


In a typical mouse PK study, you can up to save 9X the number of mice* — helping your lab better address the 3 R’s and animal welfare.


Composite PK Studies

Euthanize mouse for each time point to collect 700 µL of blood through a cardiac sample

Serial PK Studies

Collect 20 µL of blood from mouse tail vein for each time point — enabling the reuse of the animal for each data point

Eliminate compound loss and get more data per animal during PK studies – further reducing animal usage

In addition to the highly accurate and precise transfers, non-contact acoustic dispensing performed by Echo Liquid Handlers eliminates the potential for compound loss on tips making the transition to microsampling easier. Increased compound potency enables the use of miniaturized reactions, reducing the volume of plasma needed per PK study and allowing for the use of microsampling.

In a Medimmune study**, the use of the Echo Liquid Handler in peptide PK assays showed an increase of 88% data collected per animal. This gain was attributed to the elimination of peptides absorbance to tips, requiring the use of less sample and directly resulting in the reduction in animal usage.

Technology Used to Perform Study Volume of Serum Required / Time Point # of Time Points Possible / Animal
Tip-based liquid handler 40 µL 3
Echo® Acoustic Liquid Handler 5 µL 24
Technology Used to Perform Study Tip-based liquid handler
Volume of Serum Required / Time Point 40 µL
# of Time Points Possible / Animal 3
Technology Used to Perform Study Echo® Acoustic Liquid Handler
Volume of Serum Required / Time Point 5 µL
# of Time Points Possible / Animal 24

* Joyce, A.; M. Wang; R. Lawrence-Henderson et al. (2014) One Mouse, One Pharmacokinetic Profile: Quantitiave Whole Blood Sampling Serial Sampling for Biotherapeutics. Pharm Res. DO1 10.007/s1 1095-013-1286-y.

** Naylor, J.; A. Rossi; and D. Hornigold. (2016) Acoustic Dispensing Preserves the Potency of Therapeutic Peptides throughout the Entire Drug Discovery Workflow. JALA. 21(1), 90-96.

FEATURED CONTENT
DOWNLOAD BROCHURES
  • Echo Liquid Handler
    Echo®
    Liquid Handlers
    Acoustic liquid transfer technology
    DOWNLOAD
  • Access Laboratory Workstation
    Access™
    Laboratory Workstation
    Walk-up automation for Echo Liquid Handlers
    DOWNLOAD



For Research Use Only Not for use in Diagnostics