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qPCR

Reduce working volumes and lower costs

HOME | APPLICATIONS | qPCR

Reduce Working Volumes and Lower Costs

To avoid the impacts of poor liquid handling performance, researchers traditionally use larger volumes of samples and reagents to prepare qPCR assays, — raising costs and lowering throughput. Echo Liquid Handlers reverse this trend, enabling miniaturization with reaction volumes as low as 250 nL. Customers have shown that a single Echo system can replace 8 - 10 traditional liquid handlers while increasing quality and reproducibility.

Echo 525 Liquid Handler
Echo® 525 LIQUID HANDLER

Reduce Sample Preparation Costs and Eliminate Cross-contamination


Assay miniaturization with the Echo Liquid Handler lowers the cost of 384-well and 1536-well qPCR assays, and because the Echo Liquid Handler does not require tips or contact with liquids, any risk for cross-contamination is eliminated. Additionally, precise and accurate transfer of sub-microliter volumes enables the addition of concentrated samples without prior dilution. With options for integrated plate handling automation and software tailored for genomic research, Echo systems can easily scale for production environments.

FEATURED PUBLICATION

Interlaboratory Reproducibility of Droplet Digital Polymerase Chain Reaction Using a New DNA Reference Material Format

Ex Vivo Assessment of Drug Activity in Patient Tumor Cells as a Basis for Tailored Cancer Therapy

Analytical ChemistryOctober 2, 2017

Leonardo B. Pinheiro, et al.
National Measurement Institute (NMI), Sydney, Australia

Abstract

Use of droplet digital PCR technology (ddPCR) is expanding rapidly in the diversity of applications and number of users around the world. Access to relatively simple and affordable commercial ddPCR technology has attracted wide interest in use of this technology as a molecular diagnostic tool. For ddPCR to effectively transition to a molecular diagnostic setting requires processes for method validation and verification and demonstration of reproducible instrument performance. In this study, we describe the development and characterization of a DNA reference material (NMI NA008 High GC reference material) comprising a challenging methylated GC-rich DNA template under a novel 96-well microplate format. A scalable process using high precision acoustic dispensing technology was validated to produce the DNA reference material with a certified reference value expressed in amount of DNA molecules per well. An interlaboratory study, conducted using blinded NA008 High GC reference material to assess reproducibility among seven independent laboratories demonstrated less than 4.5% reproducibility relative standard deviation. With the exclusion of one laboratory, laboratories had appropriate technical competency, fully functional instrumentation, and suitable reagents to perform accurate ddPCR based DNA quantification measurements at the time of the study. The study results confirmed that NA008 High GC reference material is fit for the purpose of being used for quality control of ddPCR systems, consumables, instrumentation, and workflow.


VIEW PUBLICATION ON ACS
WEBINAR
Optimizing High-Throughput qPCR Workflows
Dr. Srinivasa Sripathi    |   Postdoctoral Fellow at Johns Hopkins Medical Institute
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SNP Genotyping Workflow

Echo® Liquid Handling
  • DNA Isolation / Purif
  • Prepared Source Plates
  • Assemble PCR Reagents and DNA Samples
  • PCR
  • Analysis
  • DNA Samples
    (384 PP)
  • Primers, Probes
    (384 PP)
  • Master Mix
    (Reservoir)

Low volume addition of costly reagents and samples to PCR plates

Key Benefits

  • Reduced operating costs through efficient assay miniaturization
  • Improved data quality with assay volumes as low as 250 nL
  • Elimination of cross-contamination
  • Integrated systems and software tailored for genomic assays
WEBINAR
Advances in High-Throughput One-Step qPCR Directly From Cells Enabled by the Echo® Liquid Handler and Roche LightCycler®
Rupal Cutting    |   Roche Applied Sciences
Tim Allison    |   Labcyte Inc.
DOWNLOAD BROCHURES
  • Genomics Research Applications
    Genomics Research
    Applications
    Empowering genomics research with assay miniaturization
    DOWNLOAD
  • Echo Liquid Handler
    Echo®
    Liquid Handlers
    Acoustic liquid transfer technology
    DOWNLOAD
  • Access Laboratory Workstation
    Access™
    Laboratory Workstation
    Walk-up automation for Echo Liquid Handlers
    DOWNLOAD
  • Labcyte Consumables
    Labcyte®
    Consumables
    Precision manufacturing, exceptional performance
    DOWNLOAD

ACCESS CONFIGURATIONS
  • Access Workstation for Genomics
    Access™
    Workstation for Genomics
    DOWNLOAD

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For Research Use Only Not for use in Diagnostics