Reduce working volumes and lower costs
To avoid the impacts of poor liquid handling performance, researchers traditionally use larger volumes of samples and reagents to prepare qPCR assays, — raising costs and lowering throughput. Echo Liquid Handlers reverse this trend, enabling miniaturization with reaction volumes as low as 250 nL. Customers have shown that a single Echo system can replace 8 - 10 traditional liquid handlers while increasing quality and reproducibility.
Assay miniaturization with the Echo Liquid Handler lowers the cost of 384-well and 1536-well qPCR assays, and because the Echo Liquid Handler does not require tips or contact with liquids, any risk for cross-contamination is eliminated. Additionally, precise and accurate transfer of sub-microliter volumes enables the addition of concentrated samples without prior dilution. With options for integrated plate handling automation and software tailored for genomic research, Echo systems can easily scale for production environments.
Analytical ChemistryOctober 2, 2017
Leonardo B. Pinheiro, et al.
National Measurement Institute (NMI), Sydney, Australia
Use of droplet digital PCR technology (ddPCR) is expanding rapidly in the diversity of applications and number of users around the world. Access to relatively simple and affordable commercial ddPCR technology has attracted wide interest in use of this technology as a molecular diagnostic tool. For ddPCR to effectively transition to a molecular diagnostic setting requires processes for method validation and verification and demonstration of reproducible instrument performance. In this study, we describe the development and characterization of a DNA reference material (NMI NA008 High GC reference material) comprising a challenging methylated GC-rich DNA template under a novel 96-well microplate format. A scalable process using high precision acoustic dispensing technology was validated to produce the DNA reference material with a certified reference value expressed in amount of DNA molecules per well. An interlaboratory study, conducted using blinded NA008 High GC reference material to assess reproducibility among seven independent laboratories demonstrated less than 4.5% reproducibility relative standard deviation. With the exclusion of one laboratory, laboratories had appropriate technical competency, fully functional instrumentation, and suitable reagents to perform accurate ddPCR based DNA quantification measurements at the time of the study. The study results confirmed that NA008 High GC reference material is fit for the purpose of being used for quality control of ddPCR systems, consumables, instrumentation, and workflow.
Low volume addition of costly reagents and samples to PCR plates