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SEQUENCING

Low cost, highly efficient library preparation

HOME | APPLICATIONS | Sequencing

Low Cost, Highly Efficient Library Preparation

Echo Liquid Handlers enable library preparation in low microliter volumes for a range of sequencing methods including Sanger, 16S, and Next-Generation Sequencing (NGS) – to dramatically reduce reagent costs, save samples, and eliminate steps — all while improving library quality and throughput.


With Sanger sequencing, BigDye reagent costs can be a limiting factor. Sequencing volumes can be reduced from 10 µL to 2.5 µL with the use of the Echo Liquid Handler for a 4-fold reduction in cost per reaction.


16S rRNA sequencing has long been the method of choice for microbiome researchers. The use of the Echo Liquid Handler allows for miniaturized 16S rRNA sequencing, and NGS reagent cost reduction also enables researchers to shift to miniaturized shotgun sequencing.

Reduce Library Prep Costs 100-Fold

Use of the Echo Liquid Handler for NGS library preparation versus conventional liquid handlers allows for up to a 100-fold reduction in reaction volumes, dramatically reducing study reagent costs.



Comparison of Liquid Handling Methods

  Manual Pipetting Echo® Liquid Handler
Amount of DNA 50 ng 0.06 – 2.0 ng
DNA volume (Rxn) 25 µL 200 nL
Library prep volume (Rxn) 25 µL 300 nL
Total volume 50 µL 0.5 µL
Reactions per kit 96 9600
Cost per reaction $72.91 $0.73
Echo 525 Liquid Handler
Echo® 525 LIQUID HANDLER

Reduce Transfer Volumes by 75% or More

When NGS sequencing programs scale, the cost of library preparation is a limiting factor. With the Echo Liquid Handler's low-volume acoustic transfer capability, researchers can significantly increase the number of libraries generated while maintaining current budgets.

USER TESTIMONIAL

“By using the Echo 525, a scale-down by 50 – 100x can be achieved with minor modifications of the protocol, leading to significant cost reduction without sacrificing data quality. Furthermore, we demonstrate the superiority of contact-free liquid transfers in molecular biology applications with regard to reproducibility and contamination prevention.”

Stephan Lorenz  |  Wellcome Trust Sanger Institute, UK


Pool Libraries for Sequencing with Greater Accuracy in Less Time

Echo Liquid Handlers can rapidly pool and normalize oligos or DNA libraries from any well of a microplate. With average transfer times of no more than a few seconds per well, Echo Liquid Handlers can reduce pooling times by more than 80% compared to traditional liquid handlers. Since Echo systems can transfer volumes as little as 25 nanoliters, high concentration libraries do not have to be diluted prior to transfer. This capability results in simultaneous normalization while pooling. Furthermore, by avoiding tip-based sample dilution, the propagation of errors from sample retention by tips is eliminated. This drastically improves the concentration accuracy of all libraries pooled.

FEATURED TECH NOTE

Echo-Enhanced SMART-Seq® v4 for RNA Sequencing

Echo-Enhanced SMART-Seq® v4 for RNA Sequencing

APP-G122

Jefferson Lai, et al.
Labcyte Inc.

Abstract

As the cost of sequencing has continued to decline by orders of magnitude in the past ten years, scientists are enabled to ask deeper and more complex questions about transcriptomics. Consequently, the variety, quantity, and demands of RNA sequencing experiments have all increased. The Clontech SMART-Seq® v4 Ultra® Low Input RNA Kit incorporates the SMART (Switching Mechanism at 5’ End of RNA Template) technology. This technology utilizes the template switching activity of reverse transcriptases to add PCR adapters directly to both the 5’ and 3’ ends of the first-strand cDNA before amplification using LD PCR. Here, we document information and results pertaining to the miniaturization of this process utilizing the Echo® 525 Liquid Handler, effectively reducing the reagent cost and processing time for the workflow, addressing current throughput needs of RNA sequencing.


VIEW TECH NOTE

Key Benefits

  • Reduced operating costs through efficient assay miniaturization
  • Improved data quality with assay volumes as low as 250 nL
  • Elimination of cross contamination
  • Integrated systems and software tailored for genomic assays



DOWNLOAD BROCHURES
  • Genomics Research Applications
    Genomics Research
    Applications
    Empowering genomics research with assay miniaturization
    DOWNLOAD
  • Echo Liquid Handler
    Echo®
    Liquid Handlers
    Acoustic liquid transfer technology
    DOWNLOAD
  • Access Laboratory Workstation
    Access™
    Laboratory Workstation
    Walk-up automation for Echo Liquid Handlers
    DOWNLOAD
  • Labcyte Consumables
    Labcyte®
    Consumables
    Precision manufacturing, exceptional performance
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  • Access Workstation for Genomics
    Access™
    Workstation for Genomics
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For Research Use Only Not for use in Diagnostics