Low-volume sequencing libraries from single cells
Echo Liquid Handlers miniaturize library preparation for whole genome and transcriptome analysis of genetic material from single cells. With precise, accurate, and non-contact transfer, Echo systems can reduce multiple displacement amplification (MDA) reactions by 75% or more. Libraries for next-generation sequencing (NGS) of RNA or DNA can be reduced to 10 µL or less.
Single-Cell Genomics (SCG) is essential for the understanding of genetic and epigenetic variations that occur throughout the life of an organism. For example, cancer research programs are relying on SCG to overcome the challenges with data derived from heterogeneous tumor samples by studying individual cell types within a population and rare cell events.
Amplification of DNA from a single cell using MDA is inherently susceptible to contamination. Exogenous DNA from tips or nozzles used during the transfer of samples or reagents into a PCR reaction cannot be resolved with UV-irradiation steps. The Echo Liquid Handler's non-contact acoustic transfer completely avoids contact with any DNA to drastically lower contamination risk. Furthermore, the precision and accuracy of transfer at low nanoliter volumes, also reduce ongoing reagent costs.
By combining the Echo Liquid Handler's low-volume acoustic transfer capability with library preparation kits for RNA or DNA sequencing, researchers can drastically increase the number of libraries generated per kit. By cutting transfer volumes by 75% or more and pooling libraries for sequencing without iterative dilutions, Echo systems can miniaturize and improve the quality of libraries prepared from single cells.