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SYNTHETIC BIOLOGY

Increase efficiency and speed while reducing costs

HOME | APPLICATIONS | DNA Assembly

Miniaturize and Accelerate DNA Assembly for Gene Synthesis

Echo Liquid Handlers integrated into an Access Workstation provide a high-throughput, fully automated system, pooling oligos, assembling constructs, and spotting colonies. Whether using the Gibson Assembly® or Golden Gate® cloning method, tipless acoustic liquid handling reduces costs, waste, and time.

Tipless acoustic liquid handling reduces costs, waste, and time

Echo 525 Liquid Handler
Echo® 525 LIQUID HANDLER

Traditional DNA assembly methods require reactions of 10-20 microliters per well. The Echo Liquid Handler can reduce the volume 100-fold, drastically cutting costs. With the ability to reliably transfer volumes as low as 2.5 nanoliters, Echo systems can extend the useful life of a primer library and eliminate the need to dilute high concentration primers, saving on storage and primer costs, and preventing dilution errors.



Low Cost, Highly Efficient Library Preparation

Use of the Echo liquid handler for library preparation versus conventional liquid handlers allows for up to a 100-fold reduction in reaction volumes, dramatically reducing study reagent costs.


In addition to miniaturization, the Echo system can rapidly pool oligos or DNA fragments from library plates. Since there is no contact with fluid and no time spend changing or washing tips, the Echo system can transfer each oligo or fragment from any well of a microplate in less than a second. This can save 10-15 hours of time in a high-throughput setting. And since Echo systems have the ability to transfer volumes as low as 2.5 nanoliters, high concentration libraries do not have to be diluted prior to transfer, resulting in simultaneous normalization while pooling.


Finally, assay performance is essential. The accuracy and precision of Echo Liquid Handlers eliminate noise from assay data. This enables better detection of small changes in microbes and other organisms.

USER TESTIMONIAL

“(The Echo) is an extremely fast instrument... a significant savings in terms of time. We’ve been able to compress pipelines that take about 12 hours to about 3 hours.”

Sunil Chandran  |  Amyris Biotechnologies

  • SLIDE LEFT
  • SLIDE RIGHT

Synthetic Biology Workflow

Echo® Liquid Handling
  • DNA Assembly
  • QC of DNA Assembly and Cloning/
    Transfection
  • Cloning and Transfection

Benefits of Echo

  • ● 100 fold reduction of reaction volumes
  • ● Increased throughput with cherry picking

Benefits of Echo

  • ● 100-fold reduction of library prep volumes
  • ● Pool / normalize in one step

Benefits of Echo

  • ● Increased throughput
  • ● Reduced costs due to miniaturization
  • Cell Dispense to Plates
  • Functional Testing

Benefits of Echo

  • ● Reduces input requirements and detection costs
  • ● Consistency of cell transfer
  • ● Robust automated system

Key Benefits

  • Reduce DNA assembly costs
  • Quickly pool DNA oligos or fragments to save hours per day
  • Eliminate primer dilutions and dilution errors
  • Eliminate the noise in assay data
VIDEO
GenomeFoundry@ISG Work Cell, NYU Langone
Genome Foundry Video
Institute for Systems Genetics
This video shows the process of analyzing yeast colonies used to assemble chromosomes in the GenomeFoundry at NYU Langone Medical Center.

FEATURED PUBLICATION

Smart DNA Fabrication Using Sound Waves:
Applying Acoustic Dispensing Technologies to Synthetic Biology

Journal of Laboratory Automation (JALA) Special Issue
April 2016


Paulina Kanigowska, Yue Shen, Yijing Zheng, Susan Rosser, and Yizhi Cai
School of Biological Sciences, University of Edinburgh

Abstract

Acoustic droplet ejection (ADE) technology uses focused acoustic energy to transfer nanoliter-scale liquid droplets with high precision and accuracy. This non-contact, tipless, low-volume dispensing technology minimizes the possibility of cross-contamination and reduces the costs of reagents and consumables. To date, acoustic dispensers have mainly been used in screening libraries of compounds. In this paper, we describe the first application of this powerful technology to the rapidly developing field of synthetic biology, for DNA synthesis and assembly at the nanoliter scale using a Labcyte Echo 550 acoustic dispenser. We were able to successfully downscale PCRs and the popular one-pot DNA assembly methods, Golden Gate and Gibson assemblies, from the microliter to the nanoliter scale with high assembly efficiency, which effectively cut the reagent cost by 20- to 100-fold. We envision that acoustic dispensing will become an instrumental technology in synthetic biology, in particular in the era of DNA foundries.


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CUSTOMER VIDEO TESTIMONIAL
Scientists from Amyris Biotechnologies Discuss the Echo® Liquid Handler
Amyris Video Testimonial
Amyris Scientists    |   in collaboration with Labcyte Inc.
DOWNLOAD BROCHURES
  • Genomics Research Applications
    Genomics Research
    Applications
    Empowering genomics research with assay miniaturization
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  • Echo Liquid Handler
    Echo®
    Liquid Handlers
    Acoustic liquid transfer technology
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  • Access Laboratory Workstation
    Access™
    Laboratory Workstation
    Walk-up automation for Echo Liquid Handlers
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  • Labcyte Consumables
    Labcyte®
    Consumables
    Precision manufacturing, exceptional performance
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Featured Publications
FEATURED CONTENT
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ACCESS CONFIGURATIONS
  • Access Workstation for Genomics
    Access™
    Workstation for Genomics
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APPLICATION NOTES
For Research Use Only Not for use in Diagnostics