The Wellcome Trust Sanger Institute is one of the premier centers of genomic discovery and understanding in the world. The Institute is part of the Wellcome Genome Campus located in Cambridge, UK. Dr. Stephan Lorenz, Head of the Single Cell Genomics Core Facility spoke at our Edinburgh Genomics Symposium about their work in single cell analysis. In his talk, Dr. Lorenz provides an overview of the workflows used at the facility for isolating cells, amplifying RNA and DNA, library preparation and sequencing. He uses a mouse embryonic stem cell case study to demonstrate their approach. This study investigated the subpopulation structure and the effects of different induced cell differentiation and pluripotency stages on the heterogeneity of gene expression. Dr. Lorenz illustrates how single cell transcriptome sequencing increases the resolution and enables cycling of cells between distinct developmental subpopulations to be observed.
Dr. Lorenz presents data demonstrating how acoustic liquid handling reliably transfers DNA with wide ranging sizes and concentrations. He also describes how the technology can routinely quantify eight 384-well plates, in one run with standards and normalization. The group uses the Nextera method for DNA sample preparation and have adapted and miniaturized these protocols for the Echo Liquid Handler to eliminate issues of high tip consumption and high cost per sample, while maintaining the same performance quality. The team is currently extending this to accommodate a 1536-well plate format. In addition, the they have simplified the final stages of library construction and QC using BioAnalyser qPCR and acoustic liquid handling, reducing reaction volumes 10-fold.
Read a full summary of Dr. Lorenz research » WATCH THE PRESENTATION