Home

Applications

APPLICATIONS OVERVIEW
DRUG DISCOVERY
ADME-Tox
Biochemical Assays
Cell-based Assays
HTS and Secondary Screening
Microsampling
Protein Crystallography
RNAi
Sample Management
CANCER RESEARCH
Personalized Medicine
Functional Screening / DSRT
RNAi
Sequencing
Single-cell Genomics
GENOMIC RESEARCH
Gene Expression
Genotyping
Microbiome
qPCR
Sequencing
Single-cell Genomics
Synthetic Biology

Products

LIQUID HANDLING
LIQUID HANDLERS OVERVIEW
Echo® 525 Liquid Handler
Echo® 555 Liquid Handler
Echo® 550 Liquid Handler
Echo® 520 Liquid Handler
AUTOMATION
AUTOMATION OVERVIEW
Access™ Laboratory Workstation
Tempo™ Automation Control Software
SOFTWARE APPLICATIONS
SOFTWARE OVERVIEW
Echo® Array Maker
Echo® Cherry Pick
Echo® Combination Screen
Echo® Compliance Manager
Echo® Dose-Response
Echo® Plate Audit
Echo® Plate Reformat
CONSUMABLES
CONSUMABLES OVERVIEW
Echo® Qualified Microplates
Labcyte® Assay Microplates
MicroClime® Environmental Lid
Echo® Qualified Reservoir

Technology

Echo® Acoustic Liquid Handling
Dynamic Fluid Analysis™
Direct Dilution

News / Events

Labcyte BLOG
Upcoming Events
Press Releases
Labcyte in the News
Labcyte Community

Resources

JALA Special Issue
Publications
Articles and Other Media
Application Notes
Posters
Webinars
Videos
Customer Profiles
Core Labs

Support

Brochures
Web Documentation*
User Guides*
Quick Start Guides*
Specification Sheets
Site Prep Guides
Service and Maintenance
Request Information

Company

About Us
Echo® Acoustic Technology
Leadership Team
Meet Labcyte
Careers
Contact and Location
Privacy Policy

@ ASHG 2017

Visit Us @ Booth 507

REGISTER FOR WORKSHOP
HOME | NEWS / EVENTS | Upcoming Events

Reduce Library Prep Costs 100-Fold

VISIT US @ ASHG 2017 in Orlando, FL, October 17 - 21, 2017, at BOOTH 507. Learn how the Echo Acoustic Liquid Handler can enable low cost, highly efficient library preparation.


Echo Liquid Handlers overcome traditional barriers in genomics by dramatically reducing sample and reagent volume requirements. While miniaturization reduces research costs, the Echo system's non-contact transfer also eliminates cross-contamination to ensure reliable and reproducible data. From the basics of PCR setup to the complexities of synthetic biology, the Echo Liquid Handler saves time and money, improves decision making, and empowers research programs.

ASHG Schedule

  • THU, OCT 19
  • Poster Presentation
  • 3:00 - 4:00 PM
  • Scaling Workflows for Growing Microbiome Applications

Location: Exhibit Hall

Speaker: Jefferson Lai, Applications Scientist, Labcyte Inc.

  • FRI, OCT 20
  • ASHG Luncheon Workshop - RSVP to Luncheon on right-side column form
  • 1:00 - 2:15 PM
  • Location: Hilton Orlando Hotel, Lake Lucerne, Lobby Level

Echo Acoustic Liquid Handling for Genomics

by Randy Dyer, Director, Product Marketing, Labcyte Inc.

NGS Library Preparation for CGT Analysis Using the Echo Liquid Handler

by Pere Mir Pardo, Ph.D., Laboratory Manager, IGENOMIX, Valencia

Abstract: The IGENOMIX Carrier Genetic Test consists of of a combined analysis of 600 Mendelian diseases by means of Next Generation Sequencing technology (NGS). The objective of CGT is to predict the genetic risk of having a child with a genetic disease by detecting the Carrier status of both members of a couple willing to conceive. CGT can be applied before attempting a pregnancy by natural means or by assisted reproduction treatment, but also before treatment with donor sperm or eggs.

When the CGT result finds a mutation in the same gene in both members of the couple, there exists a risk for their offspring to inherit a disorder related to these mutations. In those cases, Preimplantation Genetic Diagnosis (PGD) can be offered to detect these mutations in the embryos of the couple, and only embryos free of these mutations will be transferred to the maternal uterus.


Specifically, CGT involves the analysis of genetic mutations present in protein-coding regions of 547 genes (that account for 600 diseases) using a NGS-based laboratory protocol. The Echo® 525 Liquid Handler has been used in the library preparation step that is crucial for this NGS protocol. The use of this new technology in NGS library preparation allows for the reduction of the volume of reagents used per sample as well as reducing the hands-on time for this protocol. The combination of both advantages is associated with a reduction in costs.


From October 2015 through December 2016, 6,156 samples were analyzed using the conventional NGS protocol. The Echo system was introduced into our laboratory in January 2017. From then until July 2017, a total of 4,277 samples were analyzed using the Echo protocol.


Results of both protocols are similar in terms of sensitivity (0.9858) and specificity (0.9997) of mutation detection. In both cases, 5% of the couples were found to be positive for mutations in the same gene and PGD was offered to them.


In conclusion, NGS-based expanded CGT analysis constitutes a powerful tool to predict risk for Mendelian diseases, and coupled with the Echo system, it makes the test more affordable and widely available to a higher number of patients.

About Pere Mir Pardo

Pere Mir Pardo has a Degree in Sciences from the University of Valencia (Spain) and a Master’s degree in Biotechnology of Human Assisted Reproduction from the same university. His Ph.D. is related to the introduction of arrayCGH technology for clinical practice in Preimplantation Genetic Screening. He enjoys a rich experience of 10 years working in the reproductive genetics field.


He is continuously involved in projects with specific interest in the introduction of new technologies in the clinical practice of several areas in reproductive genetics: carrier genetic screening programs, non-invasive prenatal testing and preimplantation genetic analysis. He is also part of research projects in the same field. These studies have been translated into the publication of several peer-reviewed articles in international scientific journals and book chapters, along with his yearly presence as a speaker in international congresses. During 2013 and 2014, he was the Laboratory Director in IGENOMIX India (New Delhi, India), and since 2015 he is the Laboratory Manager of the Central Lab in IGENOMIX, Valencia (Spain).

DOWNLOAD BROCHURE
  • Genomics Research Applications
    Genomics Research
    Applications
    Empowering genomics research with assay miniaturization
    DOWNLOAD

POSTER PRESENTATION
RSVP Luncheon Workshop

Lunch will be served and seating is limited. To RSVP for the workshop, please complete the form below.