Wisconsin Scientists Boost Screening Throughput by Miniaturizing to 1536-Well Plates

Written by Labcyte on December 06, 2018

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Scientists from the Small Molecule Screening Facility at the University of Wisconsin, Madison, have dramatically increased their workflow throughput by incorporating 1536-well plates. This advance was made possible with the extremely low volumes that can be processed and dispensed by the Echo Liquid Handler.

For these scientists, throughput isn’t just a metric to brag about. The facility is now able to screen targets at industrial capacity from an in-house library of more than 480,000 compounds, including known compounds from the National Institutes of Health, FDA-approved drugs, and compounds from clinical trials. The low volumes also help conserve precious samples and save money on reagents. Collectively, these advantages contribute significantly to a streamlined and improved drug discovery process.

Their work was the focus of a recent webinar hosted by Drug Target Review.

Gene Ananiev, manager of the screening facility, spoke about the transition to 1536-well plates and said that miniaturizing reactions enabled “increased throughput capacity for pre-clinical drug development.”

Ananiev and his team provide resources, equipment, and expertise to researchers at the University of Wisconsin, so this accomplishment will benefit a large network of scientists interested in conducting drug discovery experiments.

In the webinar, Ananiev also described several novel techniques for screening protein expression, DNA repair mechanisms, and gene reactivation. For example, he and his team developed an FMR1-Nluc reporter hiPSC line using CRISPR/Cas9 genome editing and also demonstrated expression of the FMR1 gene by visualization of luciferase activity.

The webinar lasts for about 45 minutes and covers a number of useful topics, including performance tips for microplate readers using high-density formats. If you have time to watch it, we think you’ll find it very informative!

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