for medium throughput screening and large genomics projects
Acoustic liquid handling is changing how researchers are doing science. The precise nL transfer volumes, the any-well-to-any-well transfer capability, and flexible applications software of the Echo 550 Liquid Handler enables researchers to set up experiments not possible with traditional liquid handling.
Watch the Echo acoustic droplet ejection (ADE) technology in action. Sound waves eject precisely-sized droplets from the source plate onto the destination plate suspended above.
The high-performance fluid transfer of Echo 550 Liquid Handler delivers comparable transfer efficiency, knock-down, and cell morphology reactions to traditional liquid handlers with less use of sample and reagent.
FIGURE 1: Fluorescence image reveals KYSE cells successfully transfected with siGLO(GE Healthcare) (pink), transferred using the Echo Liquid Handler1
FIGURE 2: Inclusion intensities of esophageal cancer cells transfected using the Echo Liquid Handler vs. manual transfer. Error bars indicate standard deviations.1
High assay performance at miniaturized volumes enables researchers to move quantitative assays into higher density formats. Assays such as time-dependent inhibition perform comparably to traditional volumes with less reagent, compound, and the elimination of workflow steps.
FIGURE 3: Time-dependent CYP3A4 inhibition profiling with two inhibitors.2
FIGURE 4: Time-dependent CYP1A2 inhibition profiling with two inhibitors2
|Drop volume||2.5 nL|
|Volume transfer range||2.5 nL – 10 µL (dependent on fluid type, assay, and source plate used)|
|Reagents supported||DMSO, PCR and qPCR reagents, synthetic biology reagents, NGS reagents, primers and probes, cell culture media, proteins, nucleic acids, up to 50% glycerol, serum, plasma, antibodies, enzymes, and protein crystallography reagents|
|Transfer accuracy||<10% deviation from target volume|
|Transfer precision||<8% CV (except protein crystallography fluid class)|
|Source labware compatibility||All Echo Qualified 384- and 1536-well Microplates|
|Destination labware compatibility||All Echo Qualified Microplates and most ANSI-compliant/SBS-standard microplates in 96-, 384-, 1536-, and 3456-well formats 8 to 16 mm well height|
|Barcode reader options||Short-side and/or long-side|
|Dimensions||53.9 cm width x 68.3 cm depth x 92.5 cm height (21.2 x 26.9 x 36.4 inches)|
|Weight||128 kg (283 lbs)|
|Working envelope||Additional 25.4 cm (10 inches) on top, 2.5 cm (1 inch) on sides, 7.6 cm (3 inches) at front, 30.5 cm (12 inches) at back|
|Electrical||AC 120V, 50/60 Hz, 10 A or AC 230V, 50/60Hz, 5 A|
|Operating temperature||21°C ± 5°C (70°F ± 9°F)|
|Vacuum||House supply minimum 200 Torr (266 mbar, 22 inches Hg Vac)
Standalone vacuum with surge tank, minimum pump speed 2.0 m3/hr, minimum pressure 100 Torr (133 mbar, 26 inches Hg Vac)
|Fluid class calibration||No calibration needed|