Next generation high-throughput tube-enabled system
The Echo 655T Liquid Handler supports sample transfer directly from Echo Qualified sample tubes, enabling a fully non-contact liquid handling workflow from sample storage all the way to assay ready plates. The Echo 655T Liquid Handler is capable of transferring as little as 2.5 nL from 384-well and 1536-well microplates, and now, from sample tubes arrayed in a 96-tube format. Additionally, it transfers at the highest speed to meet the needs of high-thoughput biochemical and cell-based screening.
Watch the Echo acoustic droplet ejection (ADE) technology in action. Sound waves eject precisely-sized droplets from the source plate onto the destination plate suspended above.
Tipless, non-contact, acoustic fluid transfers eliminate the potential for samples or compounds to adhere to plastic tips. This helps to improve data quality, increasing the potential to find new therapeutics.
In a screen of 975 compounds, Bristol-Myers Squibb found 108 more hits when using the Echo Liquid Handler1.
1 Spicer T., Fitzgerald Y., Burford N., Matson S., Chatterjee M., Gilchrist M.,Myslik J., O'Connell J.
Pharmacological evaluation of different compound dilution and transfer paradigms on an enzyme assay in low volume 384-well format. Poster presented at Drug Discovery Technology, August 2005, Boston, MA.
108 | 32%
230 | 68%
Set up 384-well cell-based and biochemical assays or transition into 1536-well formats with no loss of performance. The nanoliter volume transfers of the Echo 655T systems enable direct dilution of concentrated compounds from sample tubes – eliminating workflow steps and keeping the final concentration of DMSO low in the final assay. Data accuracy is further improved through the elimination of compound loss on tips.
FIGURE 1: Stimulation curves and EC50 values for isoproterenol, salbutamol, and salmeterol in 384-well (left) and 1536-well (right), LV white microplate. Incubation time of 60 minutes. BMG Labtech PHERAstar FS.1
FIGURE 1: Stimulation curves and EC50 values for isoproterenol, salbutamol, and salmeterol in 384-well, LV white microplate. Incubation time of 60 minutes. BMG Labtech PHERAstar FS.1
FIGURE 2: Stimulation curves and EC50 values for isoproterenol, salbutamol, and salmeterol in 1536-well, LV white microplate. Incubation time of 60 minutes. BMG Labtech PHERAstar FS.1
Drug combination screening using the Echo system has revolutionized the approach to the assessment of synergistic effects of drug combinations on disease. The capability to transfer from any-well-to-any-well and the Echo Combination Screening Software make high throughput, 2-way and 3-way dose response matrix studies a reality.
|Drop volume||2.5 nL|
|Volume transfer range||2.5 nL – 5 µL (dependent on fluid type, assay, and source plate used)|
|Reagents supported||DMSO, PCR and qPCR reagents, synthetic biology reagents, NGS reagents, primers and probes, cell culture media, proteins, nucleic acids, up to 50% glycerol, serum, plasma, antibodies, enzymes, and protein crystallography reagents|
|Transfer accuracy||<10% deviation from target volume|
|Transfer precision||<8% CV (except protein crystallography fluid class)|
|Source labware compatibility||All Echo Qualified 384- and 1536-well Microplates and Echo Qualified acoustic sample tubes|
|Destination labware compatibility||All Echo Qualified Microplates and most ANSI-compliant/SBS-standard microplates in 96-, 384-, 1536-, and 3456-well formats 8 to 16 mm well height|
|Barcode reader options||Short-side and/or long-side|
|Dimensions||53.9 cm width x 68.3 cm depth x 92.5 cm height (21.2 x 26.9 x 36.4 inches)|
|Weight||130 kg (286 lbs)|
|Working envelope||Additional 25.4 cm (10 inches) on top, 2.5 cm (1 inch) on sides, 7.6 cm (3 inches) at front, 30.5 cm (12 inches) at back|
|Electrical||AC 120V, 50/60 Hz, 10 A or AC 230V, 50/60Hz, 5 A|
|Operating temperature||21°C ± 5°C (70°F ± 9°F)|
|Vacuum||House supply minimum 200 Torr (266 mbar, 22 inches Hg Vac)
Standalone vacuum with surge tank, minimum pump speed 2.0 m3/hr, minimum pressure 100 Torr (133 mbar, 26 inches Hg Vac)
|Fluid class calibration||No calibration needed|