Echo 525 LIQUID HANDLER

for high volume transfers

  • Designed for biochemical and genomic sample and reagent transfer
  • Improve data quality while saving on reagent costs
  • Reagent reservoir for larger volume reagent transfers
Description Specifications
Drop volume 25 nL
Volume transfer range 25 nL – 5 µL one well to one well
Transfer accuracy <10% deviation from target volume
Transfer precision <8% CV
Reagents supported PCR and qPCR reagents, synthetic biology reagents, NGS reagents, primers and probes, cell culture media, proteins, nucleic acids, up to 50% glycerol, serum, plasma, and antibodies
Source labware compatibility Echo Qualified 384 Polypropylene, 384 Polypropylene Plus, Reservoir, Microplates
Destination labware compatibility All Echo Qualified Microplates and most ANSI-compliant/SBS-standard microplates in 96-, 384-well formats 8 -16 mm in height. Use of 1536-well plates as destination plates is application dependent
Dimensions 53.9 cm width x 68.3 cm depth x 92.5 cm height (21.2 x 26.9 x 36.4 inches)
Weight 128 kg (283 lbs)
Working envelope Additional 25.4 cm (10 inches) on top, 2.5 cm (1 inch) on sides, 7.6 cm (3 inches) at front, 30.5 cm (12 inches) at back
Electrical AC 120V, 50/60 Hz, 10 A or AC 230V, 50/60Hz, 5 A
Operating temperature 21°C ± 5°C (70°F ± 9°F)
Vacuum House supply minimum 200 Torr (266 mbar, 22 inches Hg Vac) OR Standalone vacuum with surge tank, minimum pump speed 2.0 m3/hr (70.68 cu ft/hr), minimum pressure 100 Torr (133 mbar, 26 inches Hg Vac)
Fluid class calibration No calibration needed

Liquid Handling Reimagined

Introducing the Echo 525 Liquid Handler, the first rapid acoustic liquid handler designed specifically for biochemical and genomics reagent transfer. The Echo 525 Liquid Handler and the Echo Software Applications enable scientists to spend more time looking at data. Protocol creation and implementation in the Echo Software Applications are fast and does not require extensive training. Designed for use with the Echo System, the Access Laboratory Workstation expands the Echo 525 Liquid Handler’s utility and further frees up time.

Build Complex Assays Quickly

The flexibility, speed, accuracy, and precision of the Echo 525 Liquid Handler enable researchers to build assays that are not possible with traditional liquid handlers. The use of acoustic sound energy removes the traditional boundaries of tip and pin tool based liquid handlers. Transfer variable fluid heights and viscosities from one source plate to enable rapid assay creation in one fluid transfer run. Say goodbye to fixed plate designs as the Echo Liquid Handler moves fluids from any well to any well enabling researchers to design the experimental plates they need to make new discoveries.

Efficiently Transfer Build Reagents with Echo Qualified Reservoir

Assay assembly and the transfer of one reagent to many wells is simplified using the Echo Qualified Reservoir. With the capability of transferring up to 2.5 mL from each micro-well, you can build your assay without requiring an instrument change – saving time and money.

Key Benefits

  • Highly precise and accurate low volume transfers enabling assay miniaturization
  • Builds assays 50–100 times faster than traditional liquid handlers giving the lab higher capacity from one instrument
  • Suite of Echo Software Applications guide creation of complex protocols for a variety of applications
  • Eliminates pipetting variances that affect assay success
  • Integrates into the Labcyte Access Laboratory Workstation for increased throughput

VIDEO

The Echo Liquid Handling Technology

Echo Acoustic Liquid Handling|by Labcyte Inc.

The Echo Liquid Handler revolutionizes liquid handling with acoustic energy. Sound waves eject precisely sized droplets from a source onto a microplate, slide or other surface suspended above the source.

Flexible and Reliable Transfer of Genomics Reagents

The Echo 525 Liquid Handler transfers a wide range of fluids during one transfer including distilled water, buffers, nucleic acids, and reagents containing up to 50% glycerol. With its versatile fluid range, transfer volume range, any well to any well transfer capability, and powerful applications software the Echo 525 Liquid Handler supports a wide range of genomic assay workflows:

  • Gene Synthesis
  • PCR and qPCR assay set-up
  • RNA and DNA NGS sequencing workflows
  • Sanger sequencing assay set-up
  • Single-cell sample preparation

Precise Transfers in nL Increments

Sample transfer integrity is important for successful data generation. With highly precise and accurate non-contact transfers, the Echo 525 Liquid Handler introduces no cross-contamination during transfer of reagents or DNA.

FIGURE 1: G6PD amplification curves were generated using 600 nM G6PD primers, 300 nM G6Pd probes, 1X Roche RealTime ready DNA probes master mix, and 1 ng human cDNA. Results were read on a Roche LightCycler 480 system.

Echo 555 Liquid Handler

FIGURE 2: Source plate Echo Qualified 384-well Polypropylene Plus Microplate to a 384-well assay plate. 1 µL puc19 in 0.1 %TE per well was transferred. Roche 1536 DNA Green Master reagent following the standard protocol and data was read on a Roche Lightcycler 480.

Volume 5 µL 7 µL 10 µL
Cp Min 23.46 23.57 22.59
Cp Max 25.69 24.68 23.22
Cp Average 24.21 24.08 22.90
SD 0.48 0.30 0.18
CV 1.99% 1.23% 0.81%

Versatile System That Supports a Wide Array of Assays and Biochemical Reagents

Biochemical assays require the transfer of multiple fluid types during the experiment. The Echo 525 Liquid Handler successfully transfers antibodies, proteins, water, buffers, and highly viscous fluids from a single source plate making assay set up easy. Any well to any well transfer capability enables the creation of complex, multistep reagent addition and the ability to transfer nanoliter volumes enables successful miniaturization of complex assays.

  • AlphaLISA assays
  • HTRF assays
  • ADP-Glo assays
  • Cell-based assays