Echo 655T LIQUID HANDLER

for ultra-high throughput screening

  • Transfer from acoustic sample tubes, 384-well and 1536-well microplates
  • Ideal for large sized small molecule compound screening programs
  • Fast, accurate, and precise liquid transfers in nanoliter volumes
Description Specifications
Drop volume 2.5 nL
Volume transfer range 2.5 nL – 5 µL (dependent on fluid type, assay, and source plate used)
Reagents supported DMSO, PCR and qPCR reagents, synthetic biology reagents, NGS reagents, primers and probes, cell culture media, proteins, nucleic acids, up to 50% glycerol, serum, plasma, antibodies, enzymes, and protein crystallography reagents
Transfer accuracy <10% deviation from target volume
Transfer precision <8% CV (except protein crystallography fluid class)
Source labware compatibility All Echo Qualified 384- and 1536-well Microplates and Echo Qualified acoustic sample tubes
Destination labware compatibility All Echo Qualified Microplates and most ANSI-compliant/SBS-standard microplates in 96-, 384-, 1536-, and 3456-well formats 8 to 16 mm well height
Barcode reader options Short-side and/or long-side
Dimensions 53.9 cm width x 68.3 cm depth x 92.5 cm height (21.2 x 26.9 x 36.4 inches)
Weight 130 kg (286 lbs)
Working envelope Additional 25.4 cm (10 inches) on top, 2.5 cm (1 inch) on sides, 7.6 cm (3 inches) at front, 30.5 cm (12 inches) at back
Electrical AC 120V, 50/60 Hz, 10 A or AC 230V, 50/60Hz, 5 A
Operating temperature 21°C ± 5°C (70°F ± 9°F)
Vacuum House supply minimum 200 Torr (266 mbar, 22 inches Hg Vac)
OR
Standalone vacuum with surge tank, minimum pump speed 2.0 m3/hr, minimum pressure 100 Torr (133 mbar, 26 inches Hg Vac)
Fluid class calibration No calibration needed

The New Sound of Sample Management

Due to their outstanding throughput, high transfer accuracy and precision down to nanoliter volumes and any-well to any-well transfer capabilities, Echo Liquid Handlers have long been integral to state-of-art sample management and high throughput screening solutions.

Offering these same benefits, the Echo 655T Liquid Handler now supports sample transfer directly from Echo Qualified sample tubes enabling a fully non-contact dispensing workflow from sample storage all the way to assay ready plates. Capable of transferring as little as 2.5 nL from 384-well and 1536-well microplates and now acoustic tubes arrayed in a 96-tube format, the Echo 655T Liquid Handler is ready to meet the needs of a wide variety of biochemical and cellular assays.

Echo 655T Liquid Handler

Key Benefits

  • Fastest dispense rate from an acoustic sample tube
  • Simplified compound library screening through direct transfer from acoustic sample tubes
  • Elimination of compound lose due to adhesion to or contamination from tip plastic through non-contact transfer
  • Minimized DMSO hydration through environment controlled Echo Plate Zone
  • Supports 384-well and 1536-well microplates in addition to new acoustic sample tube

Acoustic Sample Tube Management

The FluidX AcoustiX Sample Tube (FAST) was developed in collaboration with and is sold by Brooks Life Sciences for ultra-high-density sample storage. The acoustic tube offers the following benefits:

  • Enhanced sample integrity through unique cap design – permits over 500 cap/decap cycles
  • Random access to any stored sample minimizing freeze/thaw cycles
  • Echo Qualified Tube design and manufacturing – compatible with Echo 650T and 655T Liquid Handlers
  • Working volume of 15-90 µL and 20-90 µL with DMSO and aqueous fluids respectively

Workflow Automation

To meet the broad spectrum of sample throughput needs, the Echo 655T Liquid Handler seamlessly integrates with the highly configurable Access Dual Robot System accommodating a wide variety of devices and enabling a fully automated workflow. Whether your workflow requires plate reformatting, hit picking, daughter plate creation or adding samples and reagents for screening assays, Labcyte offers a fully integrated Echo 655T Liquid Handler solution to meet your needs.

Learn more about the Access Dual Robot System

VIDEO

The Echo Liquid Handling Technology

Echo Acoustic Liquid Handling|by Labcyte Inc.

The Echo Liquid Handler revolutionizes liquid handling with acoustic energy. Sound waves eject precisely sized droplets from a source onto a microplate, slide or other surface suspended above the source.

Impacting Therapeutic Discovery

Tipless, non-contact, acoustic fluid transfer of the Echo 655T Liquid Handler eliminates the potential for samples or compounds to adhere to tips. This helps to improve data quality, increasing the potential to find new therapeutics.

32% More Active Compounds Found

Potent by

Echo transfer

108 | 32%

Potent by

both methods

230 | 68%

In a screen of 975 compounds, Bristol-Myers Squibb found 108 more hits when using the Echo Liquid Handler1.

1 Spicer T., Fitzgerald Y., Burford N., Matson S., Chatterjee M., Gilchrist M.,Myslik J., O'Connell J.
Pharmacological evaluation of different compound dilution and transfer paradigms on an enzyme assay in low volume 384-well format. Poster presented at Drug Discovery Technology, August 2005, Boston, MA.

Save Compounds and Money – Miniaturized Cell-based Biochemical Assay

Utilize the Echo 655T Liquid Handler to set up 384-well cell-based and biochemical assays or transition into 1536-well formats with no loss of performance. The nL volume transfers of the Echo 655T systems enable direct dilution of concentrated compounds – eliminating workflow steps and keeping the final concentration of DMSO low in the final assay. Data accuracy is further improved through the elimination of compound loss on tips.

FIGURE 1: Stimulation curves and EC50 values for isoproterenol, salbutamol, and salmeterol in 384-well (left) and 1536-well (right), LV white microplate. Incubation time of 60 minutes. BMG Labtech PHERAstar FS.

  • Isoprotenol
  • Salbutamol
  • Salmeterol

FIGURE 1: Stimulation curves and EC50 values for isoproterenol, salbutamol, and salmeterol in 384-well, LV white microplate. Incubation time of 60 minutes. BMG Labtech PHERAstar FS.

EC50
Isoproterenol 0.8 nM
Salbutamol 11 nM
Salmeterol 3.2 nM
Z’ Factor 0.72
  • Isoprotenol
  • Salbutamol
  • Salmeterol

FIGURE 2: Stimulation curves and EC50 values for isoproterenol, salbutamol, and salmeterol in 1536-well, LV white microplate. Incubation time of 60 minutes. BMG Labtech PHERAstar FS.

EC50
Isoproterenol 0.8 nM
Salbutamol 27 nM
Salmeterol 0.2 nM
Z’ Factor 0.89

Making High-throughput Combination Screening a Reality

Drug combination screening using the Echo 655T Liquid Handler has revolutionized the approach to the assessment of synergistic effects of drug combinations on disease. The any-well-to-any-well fluid transfer and the Echo Combination Screening application software makes high throughput, 2-way and 3-way dose response matrix studies a reality.