APPLICATION NOTES

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Nanoliter Scale DNA Assembly Utilizing the NEBuilder HiFi Cloning Kit with the Labcyte® Echo® 525 Liquid...VIEW

G126

Nanoliter Scale DNA Assembly Utilizing the NEBuilder HiFi Cloning Kit with the Labcyte® Echo® 525 Liquid...

Jared Bailey, et. al.

ABSTRACT

Previous work has shown the ability of the Labcyte® Echo® 550 Liquid Handler to generate two-piece assemblies using the Golden Gate® and Gibson Assembly® chemistr...

2018
PDF | 951.08 KB
Modular DNA Assembly of PIK3CA Using Acoustic Liquid Transfer in Nanoliter VolumesVIEW

G125

Modular DNA Assembly of PIK3CA Using Acoustic Liquid Transfer in Nanoliter Volumes

Jared Bailey, et. al.

ABSTRACT

Previous work has shown the ability of the Labcyte® Echo® Liquid Handler to generate DNA constructs using various assembly chemistries with a miniaturized protoco...

2018
PDF | 1.22 MB
High-Throughput Microliter-Sized Cell-Free Transcription-Translation Reactions for Synthetic Biology App...VIEW

G124*

High-Throughput Microliter-Sized Cell-Free Transcription-Translation Reactions for Synthetic Biology App...

Ryan Marshall, et. al.

ABSTRACT

Cell-free transcription-translation systems (TXTL) are becoming reliable tools to rapidly prototype biochemical systems programmed with synthetic gene circuits. H...

2018
PDF | 827.23 KB
Utilization of the Labcyte MicroClime® Environmental Lid to Reduce Edge Effects in a Cell-based Prolifer...VIEW

D105

Utilization of the Labcyte MicroClime® Environmental Lid to Reduce Edge Effects in a Cell-based Prolifer...

John Lesnick, Linda Orren, Noёl Ruppert

ABSTRACT

In this study, we demonstrate the benefit of utilizing the Labcyte MicroClime Environmental Lid to reduce edge effects in a functional cell-based assay by measuri...

2018
PDF | 1.31 MB
Miniaturized 16S rRNA Amplicon Sequencing with the Labcyte Echo® 525 Liquid Handler for Metagenomic and...VIEW

G123

Miniaturized 16S rRNA Amplicon Sequencing with the Labcyte Echo® 525 Liquid Handler for Metagenomic and...

Jefferson Lai and John Lesnick

ABSTRACT

Our understanding of the microbial communities in human health, metagenomics, and metataxonomics has been growing rapidly in recent years and is being increasingl...

2017
PDF | 569.64 KB
Echo® System-Enhanced SMART-Seq® v4 for RNA SequencingVIEW

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Echo® System-Enhanced SMART-Seq® v4 for RNA Sequencing

Jefferson Lai, Anna Lehto, John Lesnick and Carl Jarman

ABSTRACT

As the cost of sequencing has continued to decline by orders of magnitude in the past ten years, scientists are enabled to ask deeper and more complex questions a...

2017
PDF | 562.56 KB
A Method to Streamline and Miniaturize Nextera™ Library Preparation for Illumina® Next-Gen SequencingVIEW

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A Method to Streamline and Miniaturize Nextera™ Library Preparation for Illumina® Next-Gen Sequencing

John Lesnick

ABSTRACT

The ongoing evolution of next-generation sequencing (NGS) technologies has reduced sequencing costs by orders of magnitude and significantly increased throughput....

2017
PDF | 362.47 KB
High-throughput Miniaturization of Cytochrome P450 Time-dependent Inhibition Screening Using the Echo® 5...VIEW

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High-throughput Miniaturization of Cytochrome P450 Time-dependent Inhibition Screening Using the Echo® 5...

Jing Wang

ABSTRACT

Early assessment of the ADMET (Absorption, Distribution, Metabolism, Excretion and Toxicity) properties of drug candidates has become a critical part of modern dr...

2017
PDF | 265.90 KB
Miniaturization of Cytochrome P450 Time-dependent Inhibition Screening Using the Echo® 555 Liquid HandlerVIEW

D102

Miniaturization of Cytochrome P450 Time-dependent Inhibition Screening Using the Echo® 555 Liquid Handler

Jing Wang

ABSTRACT

Early assessment of the ADMET (Absorption, Distribution, Metabolism, Excretion and Toxicity) properties of drug candidates has become a critical part of modern dr...

2017
PDF | 263.68 KB
Miniaturization of Multiple Displacement Amplification (MDA) of Genomes from a Single CellVIEW

G106

Miniaturization of Multiple Displacement Amplification (MDA) of Genomes from a Single Cell

Labcyte

ABSTRACT

Advances in whole-genome amplification have enabled sequencing of DNA and RNA from a single cell, or what is commonly called Single-cell Genomics (SCG). SCG has b...

2017
PDF | 281.42 KB
The reliability of assay-ready plates for cell-based screening enabled by the Echo® 550 Liquid HandlerVIEW

CUSTOMER APP NOTE

The reliability of assay-ready plates for cell-based screening enabled by the Echo® 550 Liquid Handler

Sheraz Gul and Markus Wolf

ABSTRACT

In a collaboration between the Fraunhofer Institute for Molecular Biology and Applied Ecology (Hamburg, Germany),
and the National University of Ireland (NU...

2017
PDF | 1.01 MB
Preparation of Mouse Plasma Microsamples for LC-MS/MS Analysis Using the Echo® Liquid HandlerVIEW

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Preparation of Mouse Plasma Microsamples for LC-MS/MS Analysis Using the Echo® Liquid Handler

Randy Dyer

ABSTRACT

Serial microsampling of animals reduces the overall number of animals sampled and euthanized for toxicology studies. Traditional composite studies in mice can req...

2017
PDF | 287.25 KB