APPLICATION NOTES

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Effective Miniaturization of Illumina Nextera XT Library Prep for Multiplexed Whole Genome Sequencing an...VIEW

G121*

Effective Miniaturization of Illumina Nextera XT Library Prep for Multiplexed Whole Genome Sequencing an...

Jefferson Lai, John Lesnick, Noël Ruppert

ABSTRACT

The cost of sequencing per nucleotide has been declining by orders of magnitude over the past ten years; however library preparation of samples for next-generatio...

2017
PDF | 965.62 KB
High-Precision Cell Dispensing with the Labcyte Echo® Liquid HandlerVIEW

D107

High-Precision Cell Dispensing with the Labcyte Echo® Liquid Handler

John Lesnick, Aurore Lejeune-Dodge, Noël Ruppert, Carl Jarman

ABSTRACT

In this technical note, we demonstrate the utility of the Labcyte Echo Liquid Handler to accurately dispense a variety of cell lines to ensure uniformity and viab...

2016
PDF | 1.26 MB
Miniaturized PIK3CA Mutational Analysis Utilizing the Labcyte Echo® Liquid HandlerVIEW

G115

Miniaturized PIK3CA Mutational Analysis Utilizing the Labcyte Echo® Liquid Handler

John Lesnick, Linda Orren, Noël Ruppert

ABSTRACT

By combining the acoustic liquid transfer of the Labcyte Echo Liquid Handler with real-time PCR mutation analysis, we have demonstrated a highly efficient, low-co...

2016
PDF | 1.54 MB
Miniaturized Enzo Life Sciences™ HDAC1 Fluor de Lys® Assays Using an Echo® Liquid Handler Integrated in...VIEW

G114

Miniaturized Enzo Life Sciences™ HDAC1 Fluor de Lys® Assays Using an Echo® Liquid Handler Integrated in...

Linda Orren, Bonnie Edwards, John Lesnick, Carl Peters

ABSTRACT

Epigenetic targets continue to provide alluring opportunities for drug discovery efforts in the pharmaceutical industry. Such pursuits typically involve the inves...

2016
PDF | 1.06 MB
Optimized NGS Library Preparation with Acoustic Liquid HandlingVIEW

G113

Optimized NGS Library Preparation with Acoustic Liquid Handling

Jeffrey H. Kim*, Maritza Duarte*, Howard Lee, John Lesnick

ABSTRACT

Radiant Genomics and Labcyte have developed a low-volume, multiplexed next-generation sequencing (NGS) library preparation method using the Echo® 525 Liquid Handl...

2015
PDF | 470.78 KB
Miniaturized EPIgeneous™ HTRF® Assays Using the Echo® Liquid Handler

Bonnie Edwards, Nga Tang, Maggie Nakamura

ABSTRACT

Cisbio’s HTRF assay technology has a wide range of applications in cell-based and biochemical assays. These assays are readily adaptable to high-throughput screen...

2015
PDF | 552.74 KB
Miniaturization of DNA Assembly for Synthetic BiologyVIEW

G108

Miniaturization of DNA Assembly for Synthetic Biology

Labcyte

ABSTRACT

Traditional DNA assembly methods require reactions of 10-20 microliters per well. The Echo® Liquid Handler can reduce that volume 10-fold, drastically cutting cos...

2015
PDF | 92.44 KB
High Throughput RT-qPCR for Gene Expression Analysis

Labcyte scientists

ABSTRACT

The current standard of accuracy for quantification of gene expression is reverse transcriptase quantitative PCR (RT-qPCR).1 The method offers the highest level o...

2015
PDF | 422.96 KB
Miniaturization of an Epigenetic AlphaLISA® Assay with the Labcyte® Echo® Liquid Handler and the BMG LAB...

Edwards B., Wang J., and Peters C

ABSTRACT

Epigenetics continues to emerge as an important target class for drug discovery and cancer research. As programs scale to evaluate many new targets related to epi...

2014
PDF | 1.40 MB
Lower Cost, Higher Throughput Library Preparation with the Echo Liquid Handler and the NuGEN Ovation® Si...VIEW

G107

Lower Cost, Higher Throughput Library Preparation with the Echo Liquid Handler and the NuGEN Ovation® Si...

Labcyte

ABSTRACT

In this proof of concept study (POC), the Ovation Single Cell RNA-Seq System miniaturization was validated by examining libraries prepared and sequenced at tradit...

2014
PDF | 444.48 KB
Miniaturized AlphaLISA® Assays Using the Echo® Liquid HandlerVIEW

D104

Miniaturized AlphaLISA® Assays Using the Echo® Liquid Handler

B Edwards, J Wang, C Peters, EJ Dell*

ABSTRACT

Labcyte Echo liquid handlers transfer compounds and reagents at nanoliter volumes without the use of tips or contact. This capability provides the precision and a...

2014
PDF | 359.92 KB
Filling MicroClime® Environmental Lids

Labcyte

ABSTRACT

The Labcyte® MicroClime environmental lid minimizes edge effects and preserves the concentration of solutions in microplate wells. Unlike standard microplate lids...

2014
PDF | 106.77 KB