ARTICLES and Other Media

featuring the Echo Acoustic Technology

121 Total Articles and Other Media

TITLES and AUTHORS

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  • Lower Cost, Higher Throughput Library Preparation with the Echo Liquid Handler and the NuGEN Ovation® Single Cell RNA-Seq System
  • Labcyte Inc.
  • Institution: Labcyte Inc. and BMG LABTECH GmbH
  • Publication: APPLICATION NOTE
  • 2014
  •  

In this proof of concept study (POC), the Ovation Single Cell RNA-Seq System miniaturization was validated by examining libraries prepared and sequenced at traditional and miniaturized reaction volumes. Additionally, the sample and reagent transfers were automated using the Echo® acoustic liquid handling technology. Echo liquid handlers transfer a wide range of fluids without contact of tips or recalibration between fluid types. The industry leading accuracy and precision of Echo liquid handlers at microliter and nanoliter volumes in combination with the NuGEN Ovation Single Cell RNA-Seq System increases library preparation throughput while reducing the costs. This enables a broader application of transcriptome analysis with NGS.

  • Publication / Type:APPLICATION NOTE
  • Related Subject:Lower Cost Higher Throughput Library Preparation Echo Liquid Handler NuGEN Ovation® Single Cell RNA-Seq System
  • Filling MicroClime® Environmental Lids
  • Labcyte Inc.
  • Institution: Labcyte Inc.
  • Publication: APPLICATION NOTE
  • 2014
  •  

The Labcyte® MicroClime environmental lid minimizes edge effects and preserves the concentration of solutions in microplate wells. Unlike standard microplate lids, the MicroClime lid incorporates a unique, fluid-absorbing matrix. The absorbed fluid creates a vapor barrier that protects the sample from the exterior environment, especially near the edges of the microplate. The MicroClime lid is easy to use—simply place it on or lift it off a microplate. No specialized tools are required, and it is compatible with robotic plate lidding systems. The lid can be filled with many different fluids, using either a hand-held syringe or automated bulk fillers.

When filled with anhydrous DMSO, the MicroClime lid prevents evaporation and hydration of DMSO in compound library plates during storage. It also stops evaporation of ultra-low-volume DMSO droplets in screening assays. The lid can also be filled with water to prevent evaporation in aqueous assays.

  • Publication / Type:APPLICATION NOTE
  • Related Subject:MicroClime® Environmental Lids
  • Miniaturized AlphaLISA® Assays Using the Echo® Liquid Handler
  • Edwards B, Wang J, Peters C, Dell EJ
  • Institution: Labcyte Inc., BMG LABTECH GmbH
  • Publication: APPLICATION NOTE - D104
  • 2014
  •  

Labcyte Echo liquid handlers transfer compounds and reagents at nanoliter volumes without the use of tips or contact. This capability provides the precision and accuracy to miniaturize biochemical and cell-based assays for screening in 1536-well microplates. AlphaLISA technology is a faster, no-wash alternative to traditional ELISA assays for biomarker and cytokine detection. With high sensitivity and low background, these assays can be readily adapted to miniaturization with the Echo liquid handler. To complement the performance of the Echo liquid handler, the BMG PHERAstar® FS plate reader provides excellent sensitivity and throughput for miniaturized, high density assays. In this application note, we use a TNF-α AlphaLISA assay to validate the performance of a miniaturized assay.

  • Publication / Type:APPLICATION NOTE - D104
  • Related Subject:AlphaLISA® Assays Echo® Liquid Handler
  • The Development of an Echo® Qualified Reagent Reservoir
  • E. K. Sackmann, J. Holmdahl, J. Daulton, D. Harris, L. Ghislain, M. Sonntag, B. Eaton, S. Rajeswari,
  • Institution: Labcyte Inc., BMG LABTECH GmbH
  • Publication: POSTER
  • 2014
  •  

Acoustic liquid handling utilizes high-frequency sound waves that are focused on the surface of a fluid to eject nano- and picoliter volumes with high accuracy and precision (Figure 1). The Echo® 550 and Echo® 555 liquid handlers transfer 2.5 nL droplets from 384-well and 1536-well microtiter plates, across a wide range of fluids up to 1.25 μL/s.

The new Echo® 525 liquid handler increases the transfer droplet volume 10-fold to 25 nL, which allows for transfer rates of 5 μL/s and enables workflows for life science applications that were previously less practical at slower rates. To enable the transfer of large quantities of fluid at this faster transfer rate we are developing a companion consumable, the multi-well Reagent Reservoir (RES), for use in these various applications.

  • Publication / Type:POSTER
  • Related Subject:Echo® Reagent Reservoir
  • The Advantages of Acoustic Liquid Handling for Drug Sensitivity
  • Carl Jarman, Krister Wennerberg, Bhagwan Yadav, Tea Pemovska, Tero Aittokallio, Bonnie Edwards
  • Institution: Labcyte Inc., Institute for Molecular Medicine Finland, FIMM, University of Helsinki
  • Publication: POSTER
  • 2014
  •  

Recently identified associations between variants of cancer genes and drug resistance have increased the value for comprehensive drug sensitivity screening in combination with molecular profiling of cancer cells. This in turn, has led to a demand for improvements to screening throughput and efficiency. Echo® Liquid Handlers use acoustic energy to provide high throughput, non-contact, liquid handling for a range of applications. Non-contact transfer avoids the risk of cross-contamination, eliminates tip costs, and facilitates the progression to high density assay formats. Echo Liquid handlers precisely and accurately transfer 2.5 nL droplets of sample and reagent, which enables the miniaturization of biochemical screens. This poster discusses the implementation of miniaturized drug sensitivity screening, at the Institute of Molecular Medicine in Finland (FIMM), with assay-ready plates produced by the Echo liquid handler.

  • Publication / Type:POSTER
  • Related Subject:Advantages Acoustic Liquid Handling Drug Sensitivity
  • Pipettes and Serial Dilutions Have Dramatic Negative Effects on Biological Assays
  • J. Olechno, S. Ekins, A. Williams, R. Ellson
  • Institution: Labcyte Inc., Collaborations in Chemistry, Royal Society of Chemistry
  • Publication: POSTER
  • 2014
  •  

Liquid handling and dilution processes profoundly influence estimates of biological activity. We compared tip-based serial dilution and dispensing versus acoustic dispensing with direct dilution. Published data show results differ by orders of magnitude with no correlation or ranking of datasets. Pharmacophores generated from data derived by both acoustic and tip-based transfer differ significantly. The acoustic dispensing-derived pharmacophore correctly identified active compounds in a subsequent test set where the tip-based method failed completely. Acoustic data generates a pharmacophore containing two hydrophobic features, one hydrogen bond donor and one hydrogen bond acceptor and is consistent with X-ray crystallography studies. In contrast, the tip-based data suggest a pharmacophore with two hydrogen bond acceptors, one hydrogen bond donor and no hydrophobic features. This pharmacophore is inconsistent with the X-ray crystallographic studies. In short, tip-based dispensing processes are an important source of error in high-throughput screening. These findings have far-reaching implications in biological research.

  • Publication / Type:POSTER
  • Related Subject:Dispensing Processes Impact Apparent Biological Activity as Determined by Computational and Statistical Analyses, Ekins et al., PLOS ONE, 2013
  • Miniaturized High Throughput Kinase Screening Using the Echo® Liquid Handler and Access™ Workstation
  • Jing Wang, Bonnie Edwards, and Kevin Kershner
  • Institution: Labcyte Inc., Sunnyvale, CA; Promega Corporation, Madison, WI
  • Publication: POSTER
  • 2014
  •  

Small molecule screening against kinases is commonly used to discover drug candidates that target various human diseases. The sheer number of small molecules tested and new targets identified every year drives screening programs to push the limits of throughput and efficiency. By combining the nanoliter-scale and non-contact Labcyte Echo 525 liquid handler with the robust and ultra-sensitive Promega ADP-Glo assay, high throughput screening can be improved. This poster demonstrates the reproducibility of inhibition and IC50 determinations from a high throughput screen performed with the Echo 525 liquid handler. In this miniaturized and high throughput format high quality and reliable data can be achieved with reduced sample and reagent volumes.

  • Publication / Type:POSTER
  • Related Subject:Miniaturized High Throughput Kinase Screening Echo® Liquid Handler Access™ Workstation
  • Miniaturization of AlphaLISA Assays with the Labcyte Echo® Liquid Handler and BMG PHERAstar® FS
  • Bonnie Edwards, Jing Wang, Carl Peters, E.J. Dell
  • Institution: Labcyte Inc., Sunnyvale, CA, USA; BMG LABTECH GmbH, Ortenberg, Germany
  • Publication: POSTER
  • 2014
  •  

PerkinElmer AlphaLISA® technology is a faster, no-wash alternative to traditional ELISA assays for biomarker and cytokine detection. With high sensitivity and low background, these assays can be miniaturized to low volumes and adapted to automation. Labcyte Echo liquid handlers transfer compounds and reagents at nanoliter volumes without the use of tips or contact. This capability provides precision and accuracy to miniaturize biochemical assays for screening in 1536-well microplates. To complement the performance of Echo liquid handlers, the BMG PHERAstar FS plate reader provides excellent sensitivity and throughput for miniaturized, high density assays.

  • Publication / Type:POSTER
  • Related Subject:Miniaturization of AlphaLISA Assays Labcyte Echo® Liquid Handler BMG PHERAstar® FS
  • Direct Improvement with Direct Dilution
  • Joe Olechno, Sean Ekins, Anthony J. Williams, Mark Fischer-Colbrie
  • Institution: Labcyte Inc.
  • Publication: American Laboratory
  • 2013
  •  

The advent of a new technology often brings far-reaching benefits well beyond the initial perception of its key advantages. Acoustic dispensing, for example, with the ability to have highly controlled droplet transfers at 2.5nL increments with exquisite precision and accuracy, does much more than save money by eliminating pipets. It enables new work flows, including those in which multiple pharmaceutical firms have seen dramatic differences in the true success or failure of compounds. This article shows how the use of acoustic dispensing and direct dilution leads to the creation of better compound structures and the potential for faster development of new drugs.

  • Publication / Type:American Laboratory
  • Related Subject:direct dilution, american laboratory, serial dilution, astrazeneca
  • Impact of Acoustic Dispensing on Data Quality in HTS and Hit Confirmation
  • Wingfield J
  • Institution: AstraZeneca
  • Publication: POSTER
  • 2013
  •  

The quality of data generated within primary screening is dependant on three things: the quality of the compounds, the quality of the biology in the test well and the quality of data analysis.Traditionally compounds are diluted from concentrated stocks using aqueous diluents before testing in primary assays. This reduces the amount of organic solvent in the assay while lowering the compound concentration. However, reducing the concentration of solvent can allow the compound to drop out of solution. Acoustic Droplet Ejection (ADE) technology allows for nanolitre volumes of reagents to be transferred into assay wells. This removes the requirement for intermediate dilution of stocks and ensures that the compounds are held in optimum solvent conditions prior to assay. To investigate the impact of ADE on primary screening, 10,000 samples from the AZ screening collection were dispensed using ADE and tested in a primary screening assay. This data was then directly compared with the original primary screening data generated using aqueous dilutions and traditional contact dispensers.

  • Publication / Type:POSTER
  • Related Subject:Acoustic Dispensing HTS and Hit Confirmation