POSTERS AND PRESENTATIONS

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Echo® 525 Liquid Handler-Enabled Bioassay: Miniaturized Humira / TNF L929 Cytotoxicity Bioassay

Jared Bailey and John Lesnick

ABSTRACT

The tumor necrosis factor receptor 1 (TNFR1) is a major target for drug development as a receptor for tumor necrosis factor-α (TNFα), which is a pro-inflammatory cytokine overexpressed in autoimmu...

2019
PDF | 695.57 KB
Acoustic Droplet Ejection of Human Genomic DNA Enabling Highly Precise and Highly Reproducible qPCR

Carson Riche, et al.

ABSTRACT

Acoustic droplet ejection (ADE) is a fluid dispensing technology, whereby high-frequency sound waves are focused on the surface of a fluid to eject nano- and pico-liter volumes with high accuracy a...

2019
PDF | 1.56 MB
Acoustic Mass Spectrometry for Ultra-Fast Reaction Triage of High Throughput Chemistry

Deepshikha Angrish, et al.

ABSTRACT

In this work, a de novo MS-based analysis, Acoustic Mist Ionization Mass Spectrometry (AMI-MS), has been validated for semi-quantitative analysis of crude reaction mixtures. The analysis of arrays...

2019
PDF | 1.62 MB
Intra-well Imaging of Fluid Meniscus and Mass Spectra via AMI-MS

Eric Hall, et al.

ABSTRACT

Acoustic mist ionization (AMI) enables high-throughput and high-sensitivity mass spectrometry (MS) analysis by ionizing and desolvating microdroplets (1 – 10 μm in diameter) generated via acoustic...

2019
PDF | 6.40 MB
Echo® System-Enhanced SMART-Seq v2 for RNASeq Applications

John Fuller, et al.

ABSTRACT

As next-generation sequencing costs have continued to decline by orders of magnitude in the past ten years, researchers are enabled to ask more questions about the transcriptome, with greater degre...

2019
PDF | 931.24 KB
A High Throughput Mass Spectrometry Plate Reader: Acoustic Droplet Ejection to an Open-Port Probe Sampling Interface

Lucien Ghislain, et al.

ABSTRACT

A truly label-free, ultra high-throughput screening technology is the ‘holy grail’ platform for drug discovery. Label-free LC/MS based screening technology is routinely used in pharmaceutical indus...

2019
PDF | 5.22 MB
Acoustic Single Cell Genomic Workflows

Marsha Blauwkamp, et al.

ABSTRACT

Many biological experiments are performed on “pooled cell populations”, with the key assumption being that cells of a particular “type” exhibit similar behavior and thus similar cell function. Howe...

2019
PDF | 1.61 MB
One-Step Heat Fragmentation of Human Genomic DNA Enables Acoustic Transfer

Marsha Blauwkamp, et al.

ABSTRACT

DNA is the most commonly used analyte in basic research and clinical diagnostic applications. DNA solutions are naturally occurring viscoelastic polymeric fluids. Highly concentrated, large-fragmen...

2019
PDF | 1.98 MB
dsDNA Quantitation with the Labcyte® Echo® 525 Liquid Handler for Miniaturized Reaction Volumes, Reduced Sample input, and Cost Savings

Rabia Khan, et al.

ABSTRACT

Precise dsDNA quantitation is a requirement for many workflows in genomic research. A variety of quantitation kits are commercially available for diagnostics, synthetic biology, and next-generation...

2019
PDF | 2.23 MB
Host Cell Residual DNA Testing in Reduced Volume qPCR Reactions Using Acoustic Liquid Handling

Kristie Wood, et al.

ABSTRACT

The removal of host cell impurities is a critical step in the production of biopharmaceutical products. One impurity targeted for clearance during the process is residual DNA arising from host cell...

2019
PDF | 1.39 MB
Combining Tube-based Storage with New Acoustic Liquid Handling Technology to Dramatically Increase Sample Management Efficiency

ABSTRACT

Traditional sample management and high throughput screening has relied heavily on high density microplates for compound storage and tip-based liquid handling solutions for processing. Drawbacks of...

2019
PDF | 4.89 MB
High Throughput Drug Residence Time Screening Using -Jump Dilution- Kinetics

Jared Head, et al.

ABSTRACT

In drug discovery, in vitro assessment of compound affinity is often quantified by endpoint parameters such as IC50. However, drug efficacy is also influenced by duration of the m...

2018
PDF | 229.39 KB