POSTERS AND PRESENTATIONS

PLEASE NOTE: For some posters, we require you to register or login to a Labcyte user account before downloading. Thank you.

A Miniaturized and Automated P450-Glo™ Screening System Using the Echo® Liquid Handler (with Prom...

J Wang, J Barco, T Worzella, K Kershner

ABSTRACT

Early assessment of cytochrome P450 (CYP) inhibition has become an essential component of drug discovery screening. The CYP3A4 enzyme is the most abundant enzyme in the liver and plays a major rol...

2012
PDF | 489.42 KB
Miniaturized GloSensor™ cAMP Assay Using the Echo® Liquid Handler (with Promega Corp.)

T Worzella, T Allison, P Stecha, K Kershner, M Cong, C Zimprich, C Cowan

ABSTRACT

As biological assays used for HTS and uHTS are miniaturized, it is critical to maintain assay sensitivity while minimizing reagent volumes and maximizing throughput. The GloSensor™ cAMP Assay provi...

2012
PDF | 1.17 MB
Validation of Low Volume 1536 “Assay Ready” Compound Plates for a Nuclear Hormone Receptor Screen

H Eriksson, J Brengdahl, A Hogner, B Becker

ABSTRACT

The predispensing of compound libraries by acoustic droplet ejection is a convenient way of preparing ”assay ready” compound plates. This method also allows for the direct (i.e. no predilution nece...

2011
PDF | 372.54 KB
Miniaturized Genotyping Reactions with the Echo® Liquid Handler

C Glazer, T Allison, R Dyer, J Barco, S Datwani

ABSTRACT

Advanced in miniaturization of genotyping assays have tremendously improved reaction throughput, but not without challenges. Efforts to reduce reagent costs and decrease sample volume requirements...

2011
PDF | 1.21 MB
Miniaturization of One-Step Gene Expression using the Access™ Laboratory Workstation and the Echo...

R Dyer, C Glazer, T Allison, S Datwani, J Barco

ABSTRACT

Quantitative PCR (qPCR) is a powerful tool used for genotyping, SNP analysis, and gene-expression studies. Quantitative detection of small mRNA expression changes facilitates the characterization o...

2011
PDF | 489.42 KB
Acoustic Liquid Handling Applied to Protein Crystallography – Miniaturizing, Formulating, Transfe...

J. Olechno, S. Datwani, B. Eaton, M. Sonntag, D. Harris, J. Barco, R. Huang, R. Stearns, R. Ellson

ABSTRACT

Acoustic liquid handling - using sound to move fluids and suspensions - has been wide accepted in the pharmaceutical high-throughput screening community and now has been applied to protein crystall...

2011
PDF | 1.14 MB
Miniaturizing RNAi Assays: Acoustic Droplet Ejection Enables Efficient and Reproducible Transfer...

C Jarman, C Edwards, E Bennett, A Bryne, A Hoff, G Bakos, D Kelleher, A Long, A Davies, S Pickett

ABSTRACT

To facilitate large-scale, high-throughput functional genomics studies using RNAi, we have developed acoustic droplet ejection (ADE) technology to meet the current demands of a successful siRNA lib...

2011
PDF | 538.39 KB
300 nL Gene Expression in a One-Step Process Directly From Cells

C Glazer, H Lee, M Sonntag, S Datwani

ABSTRACT

Recent innovations in cell lysis reagents and high throughput quantitative PCR miniaturization have allowed researchers to increase throughput while decreasing labor and reagent costs. To ensure hi...

2011
PDF | 1.17 MB
Access™ Laboratory Workstation: A Novel Approach to Quickly and Easily Add Automation to Echo® Li...

R. Dyer, B. Browning, H. Vlahos

ABSTRACT

As researchers begin to utilize the Echo liquid handler in areas new to automation, a need for a small scale automation platform that is easy to use and implement is critical. The Access Laboratory...

2011
PDF | 2.72 MB
Miniaturization of 1536-well Quantitative PCR using the Echo® Liquid Handler

C Glazer, M Sonntag, H Lee, C Jarman, S Pickett, S Datwani

ABSTRACT

Miniaturized quantitative PCR (qPCR) in 1536-well plates hold great promise for increased throughput and reagent savings. Delivering reagents to high-density plates can be challenging for conventio...

2011
PDF | 1.14 MB
Precise and Accurate Transfer of Kinase Enzymes

H Lee, S Datwani, S Pickett

ABSTRACT

Aqueous enzyme solutions have historically been problematic to dispense in tip-based liquid handlers. Enzymes are often supplied in solution containing glycerol. Glycerol increases the viscosity of...

2010
PDF | 104.80 KB
High Throughput Secondary Screening—1400 Dose-Response Curves per Hour

J Olechno, J Shieh, F Lesani, H Vlahos

ABSTRACT

We have developed a plate preparation workstation (the POD™ 810 plate assembler) that is controlled by an intellectual scheduler to increase the productivity of secondary screening labs. Dose-respo...

2008
PDF | 675.10 KB