POSTERS AND PRESENTATIONS

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The Advantages of Acoustic Liquid Handling for Drug Sensitivity Screening

C Jarman, K Wennerberg, B Yadav, T Pemovska, T Aittokallio, B Edwards

ABSTRACT

Recently identified associations between variants of cancer genes and drug resistance have increased the value for comprehensive drug sensitivity screening in combination with molecular profiling o...

2013
PDF | 626.37 KB
Next Generation Sequencing Sample Preparation Utilizing the Echo Liquid Handler

Lee H, Lee D, Chan K

ABSTRACT

The advent of Next-generation sequencing (NGS) has enabled researchers to overcome the limitations in resolution, scalability, and throughput experienced with capillary electrophoresis-based Sanger...

2013
PDF | 1.17 MB
Walk Away, Nanoscale Assay Preparation for High Throughput PCR Using the Access™ Laboratory Workstation and the Roche LightCycler® 1536 System

C Glazer, R Cutting, T Allison, R Dyer, B Nelson, J Barco, S Datwani, M Sonntag

ABSTRACT

2012
PDF | 1.18 MB
Nanoliter Scale High Throughput Protein Crystallography Screening

B Edwards, D Harris, S Datwani, B Eaton, R Huang, J Barco

ABSTRACT

Small scale protein crystallography offers tremendous advantages in screening conditions against small quantities of protein. A drawback of traditional liquid handling capabilities is reproducibili...

2012
PDF | 415.85 KB
A Miniaturized and Automated P450-Glo™ Screening System Using the Echo® Liquid Handler (with Promega Corp.)

J Wang, J Barco, T Worzella, K Kershner

ABSTRACT

Early assessment of cytochrome P450 (CYP) inhibition has become an essential component of drug discovery screening. The CYP3A4 enzyme is the most abundant enzyme in the liver and plays a major rol...

2012
PDF | 489.42 KB
Miniaturized GloSensor™ cAMP Assay Using the Echo® Liquid Handler (with Promega Corp.)

T Worzella, T Allison, P Stecha, K Kershner, M Cong, C Zimprich, C Cowan

ABSTRACT

As biological assays used for HTS and uHTS are miniaturized, it is critical to maintain assay sensitivity while minimizing reagent volumes and maximizing throughput. The GloSensor™ cAMP Assay provi...

2012
PDF | 1.17 MB
Validation of Low Volume 1536 “Assay Ready” Compound Plates for a Nuclear Hormone Receptor Screen

H Eriksson, J Brengdahl, A Hogner, B Becker

ABSTRACT

The predispensing of compound libraries by acoustic droplet ejection is a convenient way of preparing ”assay ready” compound plates. This method also allows for the direct (i.e. no predilution nece...

2011
PDF | 372.54 KB
Miniaturized Genotyping Reactions with the Echo® Liquid Handler

C Glazer, T Allison, R Dyer, J Barco, S Datwani

ABSTRACT

Advanced in miniaturization of genotyping assays have tremendously improved reaction throughput, but not without challenges. Efforts to reduce reagent costs and decrease sample volume requirements...

2011
PDF | 1.21 MB
Miniaturization of One-Step Gene Expression using the Access™ Laboratory Workstation and the Echo® Liquid Handler

R Dyer, C Glazer, T Allison, S Datwani, J Barco

ABSTRACT

Quantitative PCR (qPCR) is a powerful tool used for genotyping, SNP analysis, and gene-expression studies. Quantitative detection of small mRNA expression changes facilitates the characterization o...

2011
PDF | 489.42 KB
Acoustic Liquid Handling Applied to Protein Crystallography – Miniaturizing, Formulating, Transferring, Seeding, Monitoring, & LCP Formation

J. Olechno, S. Datwani, B. Eaton, M. Sonntag, D. Harris, J. Barco, R. Huang, R. Stearns, R. Ellson

ABSTRACT

Acoustic liquid handling - using sound to move fluids and suspensions - has been wide accepted in the pharmaceutical high-throughput screening community and now has been applied to protein crystall...

2011
PDF | 1.14 MB
Miniaturizing RNAi Assays: Acoustic Droplet Ejection Enables Efficient and Reproducible Transfer of siRNA Molecules at Low Concentrations

C Jarman, C Edwards, E Bennett, A Bryne, A Hoff, G Bakos, D Kelleher, A Long, A Davies, S Pickett

ABSTRACT

To facilitate large-scale, high-throughput functional genomics studies using RNAi, we have developed acoustic droplet ejection (ADE) technology to meet the current demands of a successful siRNA lib...

2011
PDF | 538.39 KB
300 nL Gene Expression in a One-Step Process Directly From Cells

C Glazer, H Lee, M Sonntag, S Datwani

ABSTRACT

Recent innovations in cell lysis reagents and high throughput quantitative PCR miniaturization have allowed researchers to increase throughput while decreasing labor and reagent costs. To ensure hi...

2011
PDF | 1.17 MB