POSTERS AND PRESENTATIONS

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Miniaturization of One-Step Gene Expression using the Access™ Laboratory Workstation and the Echo® Liquid Handler

R Dyer, C Glazer, T Allison, S Datwani, J Barco

ABSTRACT

Quantitative PCR (qPCR) is a powerful tool used for genotyping, SNP analysis, and gene-expression studies. Quantitative detection of small mRNA expression changes facilitates the characterization o...

2011
PDF | 489.42 KB
Acoustic Liquid Handling Applied to Protein Crystallography – Miniaturizing, Formulating, Transferring, Seeding, Monitoring, & LCP Formation

J. Olechno, S. Datwani, B. Eaton, M. Sonntag, D. Harris, J. Barco, R. Huang, R. Stearns, R. Ellson

ABSTRACT

Acoustic liquid handling - using sound to move fluids and suspensions - has been wide accepted in the pharmaceutical high-throughput screening community and now has been applied to protein crystall...

2011
PDF | 1.14 MB
Miniaturizing RNAi Assays: Acoustic Droplet Ejection Enables Efficient and Reproducible Transfer of siRNA Molecules at Low Concentrations

C Jarman, C Edwards, E Bennett, A Bryne, A Hoff, G Bakos, D Kelleher, A Long, A Davies, S Pickett

ABSTRACT

To facilitate large-scale, high-throughput functional genomics studies using RNAi, we have developed acoustic droplet ejection (ADE) technology to meet the current demands of a successful siRNA lib...

2011
PDF | 538.39 KB
300 nL Gene Expression in a One-Step Process Directly From Cells

C Glazer, H Lee, M Sonntag, S Datwani

ABSTRACT

Recent innovations in cell lysis reagents and high throughput quantitative PCR miniaturization have allowed researchers to increase throughput while decreasing labor and reagent costs. To ensure hi...

2011
PDF | 1.17 MB
Access™ Laboratory Workstation: A Novel Approach to Quickly and Easily Add Automation to Echo® Liquid Handlers

R. Dyer, B. Browning, H. Vlahos

ABSTRACT

As researchers begin to utilize the Echo liquid handler in areas new to automation, a need for a small scale automation platform that is easy to use and implement is critical. The Access Laboratory...

2011
PDF | 2.72 MB
Miniaturization of 1536-well Quantitative PCR using the Echo® Liquid Handler

C Glazer, M Sonntag, H Lee, C Jarman, S Pickett, S Datwani

ABSTRACT

Miniaturized quantitative PCR (qPCR) in 1536-well plates hold great promise for increased throughput and reagent savings. Delivering reagents to high-density plates can be challenging for conventio...

2011
PDF | 1.14 MB
Precise and Accurate Transfer of Kinase Enzymes

H Lee, S Datwani, S Pickett

ABSTRACT

Aqueous enzyme solutions have historically been problematic to dispense in tip-based liquid handlers. Enzymes are often supplied in solution containing glycerol. Glycerol increases the viscosity of...

2010
PDF | 104.80 KB
High Throughput Secondary Screening—1400 Dose-Response Curves per Hour

J Olechno, J Shieh, F Lesani, H Vlahos

ABSTRACT

We have developed a plate preparation workstation (the POD™ 810 plate assembler) that is controlled by an intellectual scheduler to increase the productivity of secondary screening labs. Dose-respo...

2008
PDF | 675.10 KB
Acoustic Droplet Ejection Improves Dose-Response Determinations

J Olechno, R Ellson

ABSTRACT

Dose-response experiments, such as IC50 analyses, are time- and labor-intensive. They usually require multiple serial dilutions and large amounts of sample. Aqueous serial dilutions of concentrated...

2006
PDF | 152.18 KB
Compound Transfer to 3456-well Assay Plates Using The Echo®550

F Masse, S Guiral, S Kargman, C Brideau

ABSTRACT

Many of the HTS assays at Merck are performed in the 3456-well format to enable rapid screening of the sample collection and to reduce the cost of an HTS campaign. Our laboratory has successfully m...

2005
PDF | 657.59 KB