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Effective Miniaturization of Illumina Nextera XT Library Prep for Multiplexed Whole Genome Sequencing and Microbiome Applications

Jefferson Lai, John Lesnick, Noël Ruppert

Labcyte Inc.

ABSTRACT: The cost of sequencing per nucleotide has been declining by orders of magnitude over the past ten years; however library preparation of samples for next-generation sequencing remains a costly component of the process. In synthetic biology, microbial whole genome sequencing is becoming a routine process to validate genome editing, deconvolute mutagenesis campaigns, confirm high-throughput DNA construction, and survey microbiome samples.