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ABSTRACT: The ongoing evolution of next-generation sequencing (NGS) technologies has reduced sequencing costs by orders of magnitude and significantly increased throughput. As the cost of NGS has decreased, library preparation costs have become a more significant proportion of the total cost. Miniaturizing sample preparation volumes provides the opportunity for dramatic reagent cost-savings. Here, we investigate two possible approaches to increase cost-saving in library preparation workflows which are enabled by the use of the Labcyte Echo 525 Acoustic Liquid Handler.