In this webinar, Vincent Noireaux, Ph.D., and Ryan Marshall from the University of Minnesota discuss the advantages of acoustic liquid handling in cell-free transcription-translation (TXTL) reactions. TXTL is employed in biology, bioengineering, synthetic biology, and biological physics. Major applications of TXTL include biomanufacturing and prototyping DNA programs, from regulatory elements to gene circuits. The new generation of TXTL systems is user-friendly, powerful, and versatile. Combined with automated liquid dispensing, TXTL can dramatically accelerate bioengineering and the characterization of novel technologies, such as CRISPR.